Section-illumination photoacoustic microscopy for dynamic 3D imaging of microcirculation in vivo

Abstract

We developed section-illumination photoacoustic microscopy capable of dynamic in vivo imaging of microvessels as small as $30\mathrm{\mu m}$ in diameter. The section illumination improved the elevational resolution while an ultrasound array provided the in-plane axial and lateral resolutions. Using the imaging system, we monitored the wash-in dynamics of Evans Blue in the microcirculation of mouse ears at $249\mathrm{Hz}$ 2D and $0.5\mathrm{Hz}$ 3D image acquisition rates. Such observation allowed us to differentiate the arterioles from the venules. In the future, the technology may be used to study angiogenesis, diabetes-induced vascular complications, and pharmacokinetics.

© 2010 Optical Society of America

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