Abstract

A new method, to our knowledge, for real-time direct access to depth information in scanning fluorescence microscopy is reported. It is based on axially exciting the sample and detecting the phase and intensity of the emitted light. Results obtained using an axicon lens to generate a Bessel beam for excitation and a microlens array as phase detector are presented.

© 2010 Optical Society of America

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References

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2008 (2)

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

2006 (1)

2005 (1)

2002 (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

1996 (1)

S. Kikuchi, K. Sonobe, and N. Ohyama, Opt. Commun. 123, 725 (1996).
[CrossRef]

1991 (1)

1961 (1)

M. Minsky, “Microscopy apparatus,” U.S. patent 3013467 (December 19, 1961).

Ahlgren, U.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Bachmann, A. H.

Baldock, R.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Brady, M.

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Darrell, A.

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Davison, D.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Dewhirst, M.

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

Fauver, M.

Hecksher-Sorensen, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Herman, R. M.

Hill, B.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Johnson, G. A.

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

Kikuchi, S.

S. Kikuchi, K. Sonobe, and N. Ohyama, Opt. Commun. 123, 725 (1996).
[CrossRef]

Lasser, T.

Leitgeb, R. A.

Marias, K.

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Meyer, H.

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Meyer, M. G.

Minsky, M.

M. Minsky, “Microscopy apparatus,” U.S. patent 3013467 (December 19, 1961).

Nelson, A. C.

Neumann, T.

Ohyama, N.

S. Kikuchi, K. Sonobe, and N. Ohyama, Opt. Commun. 123, 725 (1996).
[CrossRef]

Oldham, M.

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

Oliver, T.

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

Patten, F. W.

Perry, P.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Rahn, J. R.

Ripoll, J.

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Ross, A.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Sakhalkar, H.

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

Seibel, E. J.

Sharpe, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Sonobe, K.

S. Kikuchi, K. Sonobe, and N. Ohyama, Opt. Commun. 123, 725 (1996).
[CrossRef]

Steinmann, L.

Villiger, M.

Wiggins, T. A.

J. Biomed. Opt. (1)

M. Oldham, H. Sakhalkar, T. Oliver, G. A. Johnson, and M. Dewhirst, J. Biomed. Opt. 13, 021113 (2008).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Opt. Commun. (1)

S. Kikuchi, K. Sonobe, and N. Ohyama, Opt. Commun. 123, 725 (1996).
[CrossRef]

Opt. Express (1)

Opt. Lett. (1)

Phys. Med. Biol. (1)

A. Darrell, H. Meyer, K. Marias, M. Brady, and J. Ripoll, Phys. Med. Biol. 53, 3863 (2008).
[CrossRef] [PubMed]

Science (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davison, Science 296, 541 (2002).
[CrossRef] [PubMed]

Other (1)

M. Minsky, “Microscopy apparatus,” U.S. patent 3013467 (December 19, 1961).

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Figures (2)

Fig. 1
Fig. 1

(a) Scheme of a simulated sample and the corresponding lenslets normalized images ( 216 × 216   px ; 1   px = 10 μ m ) for the two labeled scanning points (i) and (ii). The insets represent the intensity profile along the directions marked with arrows. (c) Optical setup: L, laser; F1 and F2, filters; L1–L5, lenses; A, axicon lens; MLA, microlens array; S, sample; I, image plane.

Fig. 2
Fig. 2

(a)–(e) Transversal cuts ( 100 × 100   px ) of the excitation beam intensity distribution for the indicated axial positions. (f) Calibration image ( 400 × 400   px ) . (g) Image of the double fluorescence layer (inset) and the lenslets image. The images are normalized.

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