Abstract

An optical projection tomography microscope (OPTM) can improve axial resolution by viewing a sample from different perspectives. Here, we report a dual-mode OPTM that can generate 3D images of single cancer cells in both absorption mode and polarization mode. Cancer cells were labeled with hematoxylin for absorption imaging and nanorods for polarization imaging. Absorption images can provide morphologic information, and polarization images can provide molecular information. The combination of molecular detection and 3D cytological cell analysis may help with early cancer diagnosis.

© 2010 Optical Society of America

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    [CrossRef]

2009

E. Boisselier and D. Astruc, Chem. Soc. Rev. 38, 1759 (2009).
[CrossRef] [PubMed]

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

A. Wax and K. Sokolov, Laser Photonics Rev. 3, 146 (2009).
[CrossRef]

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

2008

B. N. Khlebtsov, V. A. Khanadeev, and N. G. Khlebtsov, J. Phys. Chem. C 112, 12760 (2008).
[CrossRef]

N. G. Khlebtsov, Quantum Electron. 38, 504 (2008).
[CrossRef]

J. Aaron, E. Rosa, K. Travis, N. Harrison, J. Burt, M. J. Yacaman, and K. Sokolov, Opt. Express 16, 2153 (2008).
[CrossRef] [PubMed]

2007

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

2006

B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, Nano Lett. 6, 662 (2006).
[CrossRef] [PubMed]

2005

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

C. Sonnichsen and A. P. Alivisatos, Nano Lett. 5, 301 (2005).
[CrossRef] [PubMed]

2000

L. Tsang, J. A. Kong, and K. H. Ding, Scattering of Electromagnetic Waves (Wiley-Interscience, 2000).
[CrossRef]

1998

P. Torok, P. D. Higdon, and T. Wilson, Opt. Commun. 148, 300 (1998).
[CrossRef]

1988

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE Press, 1988).

Aaron, J.

Akchurin, G. G.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Alivisatos, A. P.

C. Sonnichsen and A. P. Alivisatos, Nano Lett. 5, 301 (2005).
[CrossRef] [PubMed]

Astruc, D.

E. Boisselier and D. Astruc, Chem. Soc. Rev. 38, 1759 (2009).
[CrossRef] [PubMed]

Boisselier, E.

E. Boisselier and D. Astruc, Chem. Soc. Rev. 38, 1759 (2009).
[CrossRef] [PubMed]

Burt, J.

Chan, W. C. W.

B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, Nano Lett. 6, 662 (2006).
[CrossRef] [PubMed]

Chithrani, B. D.

B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, Nano Lett. 6, 662 (2006).
[CrossRef] [PubMed]

Connor, E. E.

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Crow, M. J.

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

Ding, K. H.

L. Tsang, J. A. Kong, and K. H. Ding, Scattering of Electromagnetic Waves (Wiley-Interscience, 2000).
[CrossRef]

Fauver, M.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Ghazani, A. A.

B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, Nano Lett. 6, 662 (2006).
[CrossRef] [PubMed]

Gole, A.

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Gosavi, S. W.

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

Grant, G.

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

Harrison, N.

Higdon, P. D.

P. Torok, P. D. Higdon, and T. Wilson, Opt. Commun. 148, 300 (1998).
[CrossRef]

Javier, D. J.

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

Kak, A. C.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE Press, 1988).

Kalele, S. A.

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

Khanadeev, V. A.

B. N. Khlebtsov, V. A. Khanadeev, and N. G. Khlebtsov, J. Phys. Chem. C 112, 12760 (2008).
[CrossRef]

Khlebtsov, B. N.

B. N. Khlebtsov, V. A. Khanadeev, and N. G. Khlebtsov, J. Phys. Chem. C 112, 12760 (2008).
[CrossRef]

Khlebtsov, N. G.

B. N. Khlebtsov, V. A. Khanadeev, and N. G. Khlebtsov, J. Phys. Chem. C 112, 12760 (2008).
[CrossRef]

N. G. Khlebtsov, Quantum Electron. 38, 504 (2008).
[CrossRef]

Kong, J. A.

L. Tsang, J. A. Kong, and K. H. Ding, Scattering of Electromagnetic Waves (Wiley-Interscience, 2000).
[CrossRef]

Kortum, R. R.

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

Kozhevnikov, I. S.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Kulkarni, S. K.

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

Lemelle, A.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Meglinski, I.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Meyer, M. G.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Miao, Q.

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

Murphy, C. J.

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Mwamuka, J.

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Nelson, A. C.

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Neumann, T.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Nitin, N.

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

Patten, F. W.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Piletsky, S. A.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Provenzale, J. M.

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

Rahn, J. R.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Roblyer, D. M.

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

Rosa, E.

Seibel, E. J.

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, Opt. Express 13, 4210 (2005).
[CrossRef] [PubMed]

Slaney, M.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE Press, 1988).

Sokolov, K.

Sonnichsen, C.

C. Sonnichsen and A. P. Alivisatos, Nano Lett. 5, 301 (2005).
[CrossRef] [PubMed]

Tiwari, N. R.

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

Torok, P.

P. Torok, P. D. Higdon, and T. Wilson, Opt. Commun. 148, 300 (1998).
[CrossRef]

Tourovskaia, A.

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

Travis, K.

Tsang, L.

L. Tsang, J. A. Kong, and K. H. Ding, Scattering of Electromagnetic Waves (Wiley-Interscience, 2000).
[CrossRef]

Veksler, B.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Wax, A.

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

A. Wax and K. Sokolov, Laser Photonics Rev. 3, 146 (2009).
[CrossRef]

Wilson, T.

P. Torok, P. D. Higdon, and T. Wilson, Opt. Commun. 148, 300 (1998).
[CrossRef]

Wyatt, M. D.

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Yacaman, M. J.

AJR, Am. J. Roentgenol.

M. J. Crow, G. Grant, J. M. Provenzale, and A. Wax, AJR, Am. J. Roentgenol. 192, 1021 (2009).
[CrossRef]

Chem. Soc. Rev.

E. Boisselier and D. Astruc, Chem. Soc. Rev. 38, 1759 (2009).
[CrossRef] [PubMed]

J. Biomed. Opt.

Q. Miao, J. R. Rahn, A. Tourovskaia, M. G. Meyer, T. Neumann, A. C. Nelson, and E. J. Seibel, J. Biomed. Opt. 14, 064035 (2009).
[CrossRef]

N. Nitin, D. J. Javier, D. M. Roblyer, and R. R. Kortum, J. Biomed. Opt. 12, 051505 (2007).
[CrossRef] [PubMed]

J. Nanophotonics

S. A. Kalele, N. R. Tiwari, S. W. Gosavi, and S. K. Kulkarni, J. Nanophotonics 1, 012501 (2007).
[CrossRef]

J. Phys. Chem. C

B. N. Khlebtsov, V. A. Khanadeev, and N. G. Khlebtsov, J. Phys. Chem. C 112, 12760 (2008).
[CrossRef]

Laser Photonics Rev.

A. Wax and K. Sokolov, Laser Photonics Rev. 3, 146 (2009).
[CrossRef]

Laser Phys. Lett.

A. Lemelle, B. Veksler, I. S. Kozhevnikov, G. G. Akchurin, S. A. Piletsky, and I. Meglinski, Laser Phys. Lett. 6, 71 (2009).
[CrossRef]

Nano Lett.

C. Sonnichsen and A. P. Alivisatos, Nano Lett. 5, 301 (2005).
[CrossRef] [PubMed]

B. D. Chithrani, A. A. Ghazani, and W. C. W. Chan, Nano Lett. 6, 662 (2006).
[CrossRef] [PubMed]

Opt. Commun.

P. Torok, P. D. Higdon, and T. Wilson, Opt. Commun. 148, 300 (1998).
[CrossRef]

Opt. Express

Pattern Recognit.

M. G. Meyer, M. Fauver, J. R. Rahn, T. Neumann, F. W. Patten, E. J. Seibel, and A. C. Nelson, Pattern Recognit. 42, 141 (2009).
[CrossRef]

Quantum Electron.

N. G. Khlebtsov, Quantum Electron. 38, 504 (2008).
[CrossRef]

Small

E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, and M. D. Wyatt, Small 1, 325 (2005).
[CrossRef]

Other

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE Press, 1988).

L. Tsang, J. A. Kong, and K. H. Ding, Scattering of Electromagnetic Waves (Wiley-Interscience, 2000).
[CrossRef]

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Figures (5)

Fig. 1
Fig. 1

Schematic of optical projection tomography microscope.

Fig. 2
Fig. 2

Setup used in the simulation. The size of the nanorod is shown in the upper right corner.

Fig. 3
Fig. 3

Comparison of axial resolution in wide field and OPTM. (a) shows the axial section of 3D psf in wide field, (b) shows the axial section of 3D psf in OPTM, (c) shows the axial section of 3D psf in experiment. [Z axis is the optical axis, (a)–(c) share the same coordinates].

Fig. 4
Fig. 4

Resolutions for nanorods at different orientations. (a) shows resolution along stretch out and perpendicular to stretch out for nanorods with orientations from 0° to 90°; (b) shows the ratios of the resolutions along the two directions.

Fig. 5
Fig. 5

3D reconstructed images for A549 cells labeled with hematoxylin and nanorods. The nanoparticles appear as red clusters within the cytoplasm (gray), while the cell nucleus is shown in blue. The cell in (a) was incubated with nanorods for 4 h, while the cell in (b) was incubated for 24 h with appearance of cytotoxicity.

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