Abstract

A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method.

© 2010 Optical Society of America

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References

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2009 (1)

P. F. Almoro and S. G. Hanson, J. Eur. Opt. Soc. Rapid Publ. 4, 09002 (2009).
[CrossRef]

2008 (2)

A. Grjasnow, A. Wuttig, and R. Riesenberg, J. Microsc. 231, 115 (2008).
[CrossRef] [PubMed]

B. Kemper and G. von Bally, Appl. Opt. 47, A52 (2008).
[CrossRef] [PubMed]

2006 (3)

2005 (1)

2004 (2)

P. Ferraro, S. De Nicola, A. Finizio, G. Pierattini, and G. Coppola, Appl. Phys. Lett. 85, 2709 (2004).
[CrossRef]

S. Yang and H. Takajo, Jpn. J. Appl. Phys. Part 1 43, 5747 (2004).
[CrossRef]

2003 (1)

2001 (1)

G. Pedrini, S. Schedin, and H. Tiziani, J. Mod. Opt. 48, 1035 (2001).

1998 (1)

D. Ghiglia and M. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, 1998).

1972 (1)

R. W. Gerchberg and W. O. Saxton, Optik (Stuttgart) 35, 237 (1972).

Alferi, D.

Almoro, P. F.

P. F. Almoro and S. G. Hanson, J. Eur. Opt. Soc. Rapid Publ. 4, 09002 (2009).
[CrossRef]

Aspert, N.

Charrière, F.

Colomb, T.

Coppola, G.

P. Ferraro, S. De Nicola, A. Finizio, G. Pierattini, and G. Coppola, Appl. Phys. Lett. 85, 2709 (2004).
[CrossRef]

P. Ferraro, S. De Nicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, Appl. Opt. 42, 1938 (2003).
[CrossRef] [PubMed]

De Nicola, S.

De Petrocellis, L.

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Decaestecker, C.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Depeursinge, C.

Dubois, F.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Ferraro, P.

Finizio, A.

Gerchberg, R. W.

R. W. Gerchberg and W. O. Saxton, Optik (Stuttgart) 35, 237 (1972).

Ghiglia, D.

D. Ghiglia and M. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, 1998).

Grilli, S.

Grjasnow, A.

A. Grjasnow, A. Wuttig, and R. Riesenberg, J. Microsc. 231, 115 (2008).
[CrossRef] [PubMed]

Hanson, S. G.

P. F. Almoro and S. G. Hanson, J. Eur. Opt. Soc. Rapid Publ. 4, 09002 (2009).
[CrossRef]

Kemper, B.

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Kühn, J.

Legros, J.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Magro, C.

Marquet, P.

Monnom, O.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Osten, W.

Pedrini, G.

G. Pedrini, W. Osten, and Y. Zhang, Opt. Lett. 30, 833 (2005).
[CrossRef] [PubMed]

G. Pedrini, S. Schedin, and H. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Pierattini, G.

Pritt, M.

D. Ghiglia and M. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, 1998).

Riesenberg, R.

A. Grjasnow, A. Wuttig, and R. Riesenberg, J. Microsc. 231, 115 (2008).
[CrossRef] [PubMed]

Saxton, W. O.

R. W. Gerchberg and W. O. Saxton, Optik (Stuttgart) 35, 237 (1972).

Schedin, S.

G. Pedrini, S. Schedin, and H. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Takajo, H.

S. Yang and H. Takajo, Jpn. J. Appl. Phys. Part 1 43, 5747 (2004).
[CrossRef]

Tiziani, H.

G. Pedrini, S. Schedin, and H. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Van Ham, P.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

von Bally, G.

Wuttig, A.

A. Grjasnow, A. Wuttig, and R. Riesenberg, J. Microsc. 231, 115 (2008).
[CrossRef] [PubMed]

Yang, S.

S. Yang and H. Takajo, Jpn. J. Appl. Phys. Part 1 43, 5747 (2004).
[CrossRef]

Yourassowsky, C.

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

Zhang, Y.

Appl. Opt. (2)

Appl. Phys. Lett. (1)

P. Ferraro, S. De Nicola, A. Finizio, G. Pierattini, and G. Coppola, Appl. Phys. Lett. 85, 2709 (2004).
[CrossRef]

J. Biomed. Opt. (1)

F. Dubois, C. Yourassowsky, O. Monnom, J. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, J. Biomed. Opt. 11, 054032 (2006).
[CrossRef] [PubMed]

J. Eur. Opt. Soc. Rapid Publ. (1)

P. F. Almoro and S. G. Hanson, J. Eur. Opt. Soc. Rapid Publ. 4, 09002 (2009).
[CrossRef]

J. Microsc. (1)

A. Grjasnow, A. Wuttig, and R. Riesenberg, J. Microsc. 231, 115 (2008).
[CrossRef] [PubMed]

J. Mod. Opt. (1)

G. Pedrini, S. Schedin, and H. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Jpn. J. Appl. Phys. Part 1 (1)

S. Yang and H. Takajo, Jpn. J. Appl. Phys. Part 1 43, 5747 (2004).
[CrossRef]

Opt. Express (1)

Opt. Lett. (2)

Optik (Stuttgart) (1)

R. W. Gerchberg and W. O. Saxton, Optik (Stuttgart) 35, 237 (1972).

Other (1)

D. Ghiglia and M. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, 1998).

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Figures (2)

Fig. 1
Fig. 1

Proposed phase microscopy setup.

Fig. 2
Fig. 2

Experimental results.

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