Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

© 2010 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. I. Freund and M. Deutsch, Opt. Lett. 11, 94 (1986).
    [CrossRef] [PubMed]
  2. Y. C. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, Opt. Lett. 22, 1323 (1997).
    [CrossRef]
  3. P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
    [CrossRef] [PubMed]
  4. L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, Opt. Lett. 25, 320 (2000).
    [CrossRef]
  5. A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
    [CrossRef] [PubMed]
  6. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
    [CrossRef] [PubMed]
  7. S. J. Lin, R. J. Wu, H. Y. Tan, W. Lo, W. C. Lin, T. H. Young, C. J. Hsu, J. S. Chen, S. H. Jee, and C. Y. Dong, Opt. Lett. 30, 2275 (2005).
    [CrossRef] [PubMed]
  8. L. Fu, X. S. Gan, and M. Gu, Opt. Lett. 30, 385(2005).
    [CrossRef] [PubMed]
  9. D. Ait-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet, Opt. Express 18, 14859 (2010).
    [CrossRef] [PubMed]
  10. P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
    [CrossRef]
  11. Y. Pu, M. Centurion, and D. Psaltis, Appl. Opt. 47, A103 (2008).
    [CrossRef] [PubMed]
  12. E. Shaffer, N. Pavillon, J. Kühn, and C. Depeursinge, Opt. Lett. 34, 2450 (2009).
    [CrossRef] [PubMed]
  13. E. Shaffer, P. Marquet, and C. Depeursinge, Opt. Express 18, 17392 (2010).
    [CrossRef] [PubMed]
  14. O. Masihzadeh, P. Schlup, and R. A. Bartels, Opt. Express 18, 9840 (2010).
    [CrossRef] [PubMed]
  15. E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6994 (1999).
    [CrossRef]
  16. B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
    [CrossRef]
  17. N. Lue, G. Popescu, T. Ikeda, R. Dasari, K. Badizadegan, and M. Feld, Opt. Lett. 31, 2759 (2006).
    [CrossRef] [PubMed]
  18. U. Schnars and W. P. O. Juptner, Meas. Sci. Technol. 13, R85 (2002).
    [CrossRef]
  19. B. Rappaz, F. Charriere, C. Depeursinge, P. J. Magistretti, and P. Marquet, Opt. Lett. 33, 744 (2008).
    [CrossRef] [PubMed]

2010 (4)

2009 (1)

2008 (2)

2006 (2)

N. Lue, G. Popescu, T. Ikeda, R. Dasari, K. Badizadegan, and M. Feld, Opt. Lett. 31, 2759 (2006).
[CrossRef] [PubMed]

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

2005 (2)

2003 (1)

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

2002 (2)

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

U. Schnars and W. P. O. Juptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

2000 (1)

1999 (2)

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 38, 6994 (1999).
[CrossRef]

1997 (1)

1986 (1)

Ait-Belkacem, D.

Alfano, R. R.

Badizadegan, K.

Bartels, R. A.

Blanchard-Desce, M.

Brasselet, S.

Bredebusch, I.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Brustlein, S.

Campagnola, P. J.

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Carl, D.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Centurion, M.

Charriere, F.

Chen, J. S.

Christie, R.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Cox, G.

P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
[CrossRef]

Cuche, E.

Dasari, R.

Depeursinge, C.

Deutsch, M.

Domschke, W.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Dong, C. Y.

Feld, M.

Freund, I.

Fu, L.

Gan, X. S.

Gasecka, A.

Gu, M.

Guo, Y. C.

Harris, D.

Ho, P. P.

Hsu, C. J.

Hyman, B. T.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Ikeda, T.

Jee, S. H.

Juptner, W. P. O.

U. Schnars and W. P. O. Juptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Kable, E.

P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
[CrossRef]

Kemper, B.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Kühn, J.

Lewis, A.

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Lin, S. J.

Lin, W. C.

Liu, F.

Lo, W.

Loew, L. M.

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Lue, N.

Magistretti, P. J.

Marquet, P.

Masihzadeh, O.

Mertz, J.

Moreaux, L.

Munhoz, F.

Nikitin, A. Y.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Pavillon, N.

Popescu, G.

Psaltis, D.

Pu, Y.

Rappaz, B.

Sacks, P.

Sandre, O.

Savage, H.

Schäfer, M.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Schantz, S.

Schlup, P.

Schnars, U.

U. Schnars and W. P. O. Juptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Schnekenburger, J.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Shaffer, E.

Sheppard, C. J. R.

P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
[CrossRef]

Tan, H. Y.

Tromberg, B. J.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

von Bally, G.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Webb, W. W.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Wei, M. D.

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Williams, R. M.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Wu, R. J.

Xu, P.

P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
[CrossRef]

Yeh, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Young, T. H.

Zhadin, N.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Zoumi, A.

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

Appl. Opt. (2)

Biophys. J. (1)

P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Chinese Optics Letters (1)

P. Xu, E. Kable, C. J. R. Sheppard, and G. Cox, Chinese Optics Letters 8, 213 (2010).
[CrossRef]

J. Biomed. Opt. (1)

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Meas. Sci. Technol. (1)

U. Schnars and W. P. O. Juptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Opt. Express (3)

Opt. Lett. (8)

Proc. Natl. Acad. Sci. USA (2)

A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99, 11014 (2002).
[CrossRef] [PubMed]

W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef] [PubMed]

Supplementary Material (1)

» Media 1: JPG (291 KB)     

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (2)

Fig. 1
Fig. 1

Experimental setup schematics: BS, beam splitter; BE, beam expander; DL, optical delay line; C, condenser lens; S, specimen; MO, microscope objective; M, mirror; TL, tube lens; F, bandpass filter; L, lens; FDC, β-barium borate frequency doubler crystal.

Fig. 2
Fig. 2

Mouse tail dermis and epidermis (bottom left corner). (a) Bright field image. (b) Cross-polarization image showing phase retardation caused by the birefringence of collagen. (c), (d) SHG amplitude (normalized) and phase (wrapped) reconstructed from a single hologram. All images present the same region of the specimen and scale bars are 10 μ m . Media 1, pseudocolor overlay of (b) and (c).

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

φ = z I z SHG 2 π λ n ( z , λ ) d z + z SHG z F 2 π λ / 2 n ( z , λ / 2 ) d z ,

Metrics