Abstract

We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.

© 2010 Optical Society of America

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References

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2010 (2)

2009 (2)

Z. Xie, S. Jiao, H. F. Zhang, and C. A. Puliafito, Opt. Lett. 34, 1771 (2009).
[CrossRef] [PubMed]

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

2007 (1)

H.F. Zhang, K. Maslov, and L. V. Wang, Nat. Protoc. 2, 797 (2007).
[CrossRef] [PubMed]

2006 (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

2002 (1)

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

2001 (1)

F. C. Delori, D. G. Goger, and C. K. Dorey, Invest. Ophthalmol. Visual Sci. 42, 1855 (2001).

1986 (1)

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

Cideciyan, A. V.

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

Delori, F. C.

F. C. Delori, D. G. Goger, and C. K. Dorey, Invest. Ophthalmol. Visual Sci. 42, 1855 (2001).

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

Dorey, C. K.

F. C. Delori, D. G. Goger, and C. K. Dorey, Invest. Ophthalmol. Visual Sci. 42, 1855 (2001).

F., H.

Fawzi, A.

Fitch, K. A.

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

Gibbs, D.

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

Goger, D. G.

F. C. Delori, D. G. Goger, and C. K. Dorey, Invest. Ophthalmol. Visual Sci. 42, 1855 (2001).

Hollyfield, J. G.

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

Hu, J.

Jacobson, S. G.

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

Jiang, M.

Jiao, S.

Liu, T.

Marmorstein, A. D.

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

Marmorstein, L. Y.

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

Maslov, K.

H.F. Zhang, K. Maslov, and L. V. Wang, Nat. Protoc. 2, 797 (2007).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Puliafito, C. A.

Puliafito, Carmen A.

S., K. Kirk

Sakagucbi, H.

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Wang, J.

Wang, L. V.

H.F. Zhang, K. Maslov, and L. V. Wang, Nat. Protoc. 2, 797 (2007).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Wei, Q.

Weiter, J. J.

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

Williams, D. S.

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

Wing, G. L.

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

Xie, Z.

Zhang, F.

H.F. Zhang, K. Maslov, and L. V. Wang, Nat. Protoc. 2, 797 (2007).
[CrossRef] [PubMed]

Zhang, H. F.

Zhou, Q.

Invest. Ophthalmol. Visual Sci. (4)

J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, Invest. Ophthalmol. Visual Sci. 27, 145 (1986).

F. C. Delori, D. G. Goger, and C. K. Dorey, Invest. Ophthalmol. Visual Sci. 42, 1855 (2001).

A. D. Marmorstein, L. Y. Marmorstein, H. Sakagucbi, and J. G. Hollyfield, Invest. Ophthalmol. Visual Sci. 43, 2435(2002).

D. Gibbs, A. V. Cideciyan, S. G. Jacobson, and D. S. Williams, Invest. Ophthalmol. Visual Sci. 50, 4386 (2009).
[CrossRef]

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Nat. Protoc. (1)

H.F. Zhang, K. Maslov, and L. V. Wang, Nat. Protoc. 2, 797 (2007).
[CrossRef] [PubMed]

Opt. Express (2)

Opt. Lett. (1)

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Figures (3)

Fig. 1
Fig. 1

Schematic of the experimental system: laser, frequency-doubled Q-switched Nd:YAG laser; ND, neutral density filter; PD, photodiode; I, iris; SMF, single-mode optical fiber; L1-4, lenses; DM, dichroic mirror; LF, longpass filter; APD, avalanche photodiode; UT, ultrasonic transducer.

Fig. 2
Fig. 2

Comparison of the PAM and AF images acquired simultaneously from a human RPE slides: (a) PAM image; (b) AF image; (c) from a commercial fluorescence microscope; (d) from bright-field microscopy. Bar: 100 μm .

Fig. 3
Fig. 3

PAM and AF images of the ciliary body of a pig eye: (a) PAM image; (b) AF image; (c) fluorescence microscopy photograph; (d) overlaid PAM and AF image where lipofuscin-like fluorophore is pseudocolored green. Bar: 100 µm .

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