Abstract

A completely numerical method, named digital self-referencing holography, is described to easily accomplish a quantitative phase microscopy for microfluidic devices by a digital holographic microscope. The approach works through an appropriate numerical manipulation of the retrieved complex wavefront. The self-referencing is obtained by folding the retrieved wavefront in the image plane. The folding operation allows us to obtain the correct phase map by subtracting from the complex region of interest a flat area outside the microfluidic channel. To demonstrate the effectiveness of the method, quantitative phase maps of bovine spermatozoa and in vitro cells are retrieved.

© 2010 Optical Society of America

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2010 (5)

2009 (2)

2006 (3)

2004 (1)

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

2002 (1)

J. C. McDonald and G. M. Whitesides, Acc. Chem. Res. 35, 491 (2002).
[CrossRef] [PubMed]

2001 (1)

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

1997 (1)

T. M. Kreis and W. Jüptner, in Principles of Digital Holography, W.Jüptner and W.Osten, eds. (Academic Verlag, 1997), pp. 253–363.

Alfieri, D.

Bae, C. Y.

Balduzzi, D.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Barbastathis, G.

Bernet, S.

Bredebusch, I.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Carl, D.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Charrière, F.

Colomb, T.

Coppola, G.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Cuche, E.

De Nicola, S.

P. Ferraro, D. Alfieri, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, Opt. Lett. 31, 1405 (2006).
[CrossRef] [PubMed]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

De Petrocellis, L.

Depeursinge, C.

Di Caprio, G.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Ding, H.

Domschke, W.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Ferraro, P.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

P. Ferraro, D. Alfieri, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, Opt. Lett. 31, 1405 (2006).
[CrossRef] [PubMed]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Finizio, A.

P. Ferraro, D. Alfieri, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, Opt. Lett. 31, 1405 (2006).
[CrossRef] [PubMed]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Galli, A.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Gioffrè, M. A.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Grilli, S.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Iodice, M.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Isikman, S. O.

Jang, J.

Jüptner, W.

T. M. Kreis and W. Jüptner, in Principles of Digital Holography, W.Jüptner and W.Osten, eds. (Academic Verlag, 1997), pp. 253–363.

Kemper, B.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Khademhosseinieh, B.

Kou, S. S.

Kreis, T. M.

T. M. Kreis and W. Jüptner, in Principles of Digital Holography, W.Jüptner and W.Osten, eds. (Academic Verlag, 1997), pp. 253–363.

Kuehn, J.

Marian, A.

Marquet, P.

Maurer, C.

McDonald, J. C.

J. C. McDonald and G. M. Whitesides, Acc. Chem. Res. 35, 491 (2002).
[CrossRef] [PubMed]

McIntyre, T. J.

Montfort, F.

Oh, C.

Ozcan, A.

Park, J.-K.

Pedrini, G.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Pierattini, G.

Popescu, G.

Puglisi, R.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Rinehart, M. T.

Ritsch-Marte, M.

Saffioti, N.

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

Schafer, M.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Schedin, S.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Schnekenburger, J.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Shaked, N. T.

Sheppard, C. J. R.

Tiziani, H. J.

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

von Bally, G.

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

Waller, L.

Wax, A.

Whitesides, G. M.

J. C. McDonald and G. M. Whitesides, Acc. Chem. Res. 35, 491 (2002).
[CrossRef] [PubMed]

Ye, J. C.

Acc. Chem. Res. (1)

J. C. McDonald and G. M. Whitesides, Acc. Chem. Res. 35, 491 (2002).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

G. Di Caprio, M. A. Gioffrè, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, IEEE J. Sel. Top. Quantum Electron. 16, 833 (2010).
[CrossRef]

J. Biomed. Opt. (1)

B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, J. Biomed. Opt. 11, 034005 (2006).
[CrossRef]

J. Mod. Opt. (1)

G. Pedrini, S. Schedin, and H. J. Tiziani, J. Mod. Opt. 48, 1035 (2001).

Meas. Sci. Technol. (1)

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, Meas. Sci. Technol. 15, 529 (2004).
[CrossRef]

Opt. Express (2)

Opt. Lett. (6)

Other (1)

T. M. Kreis and W. Jüptner, in Principles of Digital Holography, W.Jüptner and W.Osten, eds. (Academic Verlag, 1997), pp. 253–363.

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic view of the set-up; (b) wrapped phase distribution calculated by standard DH procedure; (c) pseudo-3D of the phase map.

Fig. 2
Fig. 2

The reconstructed in-focus intensity map of (a) the original wavefront and (b) its shifted replica. (c) Phase map obtained subtracting the phases of the original wavefront and the shifted replica. (d) The 2D and the pseudo-3D unwrapped phase map after the elimination of the superimposed plane.

Fig. 3
Fig. 3

In-focus image of a mouse cell line divided into (a) four regions and (b) digitally shifted. (c) Difference between the wrapped phase maps relative to (a) and (b). Phase distribution relative of (d) region 3, (e) region 2, and (f) region 4 in (a).

Fig. 4
Fig. 4

QPM calculated by (a) the double exposure approach and by (b) digital self-referencing. (c) Plot along segment AB in (a) and (b) and their difference.

Equations (3)

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φ ( n , m ) = φ 0 ( n , m ) + j k 2 R [ ( n N 0 ) 2 + ( m M 0 ) 2 ] ,
φ ref ( n , m ) = j k 2 R [ ( n N 0 + Δ N ) 2 + ( m M 0 + Δ M ) 2 ] ,
φ ( n , m ) φ ref ( n , m ) = φ 0 ( n , m ) + j k R [ Δ N ( n ( N 0 Δ N 2 ) ) + Δ M ( m ( M 0 Δ M 2 ) ) ] ,

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