Abstract

We demonstrate a scheme for efficient coherent anti-Stokes Raman scattering (CARS) microscopy free of nonresonant background. Our method is based on a compact Er:fiber laser source. Impulsive excitation of molecular resonances is achieved by an 11fs pulse at 1210nm. Broadband excitation gives access to molecular resonances from 0cm1 up to 4000cm1. Time-delayed narrowband probing at 775nm enables sensitive and high-speed spectral detection of the CARS signal free of nonresonant background with a resolution of 10cm1.

© 2010 Optical Society of America

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