Abstract

In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo–based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model.

© 2010 Optical Society of America

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2010 (7)

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

J. Chen, V. Venugopal, F. Lesage, and X. Intes, Opt. Lett. 35, 2121 (2010).
[CrossRef] [PubMed]

C. D'Andrea, N. Ducros, A. Bassi, S. Arridge, and G. Valentini, Biomed. Opt. Express 1, 471 (2010).
[CrossRef]

V. Venugopal, J. Chen, and X. Intes, Biomed. Opt. Express 1, 143 (2010).
[CrossRef]

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

M. Niedre and V. Ntziachristos, Opt. Lett. 35, 369 (2010).
[CrossRef] [PubMed]

2009 (1)

2008 (2)

2006 (1)

2005 (1)

A. H. Hielscher, Curr. Opin. Biotechnol. 16, 79 (2005).
[CrossRef] [PubMed]

2002 (1)

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Ahn, S.

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

Arridge, S.

Bacskai, B. J.

Bassi, A.

Boas, D. A.

Boverman, G.

Bremer, C.

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Chen, J.

Cubeddu, R.

Cuccia, D. J.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

D'Andrea, C.

Davis, S. C.

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

Ducros, N.

Durkin, A. J.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

Dutta, J.

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

Frangioni, J. V.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

Gioux, S.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

Graves, E. E.

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Hielscher, A. H.

A. H. Hielscher, Curr. Opin. Biotechnol. 16, 79 (2005).
[CrossRef] [PubMed]

Intes, X.

Joshi, A. A.

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

Konecky, S. D.

Kumar, A. T.

Leahy, R. M.

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

Leblond, F.

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

Lee, K.

Lesage, F.

Markel, V.

Mazhar, A.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

Niedre, M.

Ntziachristos, V.

M. Niedre and V. Ntziachristos, Opt. Lett. 35, 369 (2010).
[CrossRef] [PubMed]

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Panasyuk, G. Y.

Pogue, B. W.

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

Raymond, S. B.

Ripoll, J.

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Schotland, J. C.

Tromberg, B. J.

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

Valdes, P. A.

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

Valentini, G.

Venugopal, V.

Weissleder, R.

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Yodh, A. G.

Biomed. Opt. Express (2)

Curr. Opin. Biotechnol. (1)

A. H. Hielscher, Curr. Opin. Biotechnol. 16, 79 (2005).
[CrossRef] [PubMed]

J. Biomed. Opt. (1)

A. Mazhar, D. J. Cuccia, S. Gioux, A. J. Durkin, J. V. Frangioni, and B. J. Tromberg, J. Biomed. Opt. 15, 010506(2010).
[CrossRef] [PubMed]

J. Photochem. Photobiol. B (1)

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010).
[CrossRef]

Mol. Imag. (1)

V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, Mol. Imag. 1, 82 (2002).
[CrossRef]

Opt. Express (3)

Opt. Lett. (3)

Phys. Med. Biol. (1)

J. Dutta, S. Ahn, A. A. Joshi, and R. M. Leahy, Phys. Med. Biol. 55, 2961 (2010).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) Example of wide-field pattern spanning half of the imaged volume (marked in red) along the x axis. (b) Temporal excitation and fluorescence measurements in transmission for the mouse model shown in (a).

Fig. 2
Fig. 2

(a) Phantom design showing the positions of the three fluorescence inclusions (dimensions in mm). The highlighted volume corresponds to the field of illumination, and the dots correspond to the position of the detectors. (b), (c) Normalized Born contrast for dual-view measurements for full-field excitation. (d), (e) 50% isosurface maps of reconstructed fluorescence yield using TG and cw data types. The maximum intensity projections are shown on the sides of the reconstructed volume.

Fig. 3
Fig. 3

(a) 3D volume from the CT scan showing the position of the tube in the chest cavity. (b) Coronal slice of the reconstructed volume at z = 6.5 mm . (c) Transverse slice of the volume at y = 21.5 mm .

Tables (1)

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Table 1 Comparison of the Relative Quantification and Resolution Using the Two Data Types

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