Abstract

We report the development of a miniaturized dual-optical-zone endoscope objective lens. The lens has two foci, with 0.18 and 0.50 NAs. We demonstrate multiphoton imaging with dual fields of view and resolutions using the new lens. A combination of multiphoton and single-photon microscopic imaging is also demonstrated.

© 2010 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

David M. Huland, Christopher M. Brown, Scott S. Howard, Dimitre G. Ouzounov, Ina Pavlova, Ke Wang, David R. Rivera, Watt W. Webb, and Chris Xu
Biomed. Opt. Express 3(5) 1077-1085 (2012)

Development of a versatile two-photon endoscope for biological imaging

Youbo Zhao, Hiroshi Nakamura, and Robert J. Gordon
Biomed. Opt. Express 1(4) 1159-1172 (2010)

Dual view capsule endoscopic lens design

Ming-Jen Sheu, Che-Wen Chiang, Wen-Shing Sun, Jhi-Joung Wang, and Jui-Wen Pan
Opt. Express 23(7) 8565-8575 (2015)

References

  • View by:
  • |
  • |
  • |

  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
    [Crossref] [PubMed]
  2. W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
    [Crossref]
  3. J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
    [Crossref] [PubMed]
  4. Y. Wu, Y. Leng, J. Xi, and X. D. Li, Opt. Express 17, 7907 (2009).
    [Crossref] [PubMed]
  5. L. Fu, X. Gan, and M. Gu, Opt. Express 135528 (2005).
    [Crossref] [PubMed]
  6. R. Le Harzic, M. Weinigel, I. Riemann, K. König, and B. Messerschmidt, Opt. Express 16, 20588 (2008).
    [Crossref] [PubMed]
  7. Y. Wu, J. Xi, M. J. Cobb, and X. D. Li, Opt. Lett. 34, 953 (2009).
    [Crossref] [PubMed]
  8. D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
    [Crossref] [PubMed]

2009 (2)

2008 (1)

2005 (1)

2003 (2)

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[Crossref]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[Crossref] [PubMed]

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

1986 (1)

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[Crossref] [PubMed]

Birk, D. E.

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[Crossref] [PubMed]

Cobb, M. J.

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Fu, L.

Gan, X.

Gu, M.

Jung, J. C.

König, K.

Le Harzic, R.

Leng, Y.

Li, X. D.

Messerschmidt, B.

Riemann, I.

Schnitzer, M. J.

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Trelstd, R. L.

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[Crossref] [PubMed]

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[Crossref]

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[Crossref] [PubMed]

Weinigel, M.

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[Crossref]

Wu, Y.

Xi, J.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[Crossref]

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (6)

Fig. 1
Fig. 1

(a) Ray tracing diagram of the optical properties of the bifocal lens assembly. (b) Top view of a bifocal lens assembly in a lens holder. The center and peripheral optical zones are indicated by the image circular truncation of an underlying grid pattern.

Fig. 2
Fig. 2

Schematic diagram of the experiment setup for one-photon reflectance and multiphoton imaging.

Fig. 3
Fig. 3

Reflection images of a USAF target measured with NAs of (a) 0.18 and (b) 0.5. The insets in (a) and (b) are magnified images of the bars highlighted by the square. For all images, a 1 Hz frame rate was used.

Fig. 4
Fig. 4

Fluorescence intensity axial profile (in logarithmic scale) of both foci through the center of a fluorescence bead along the axial direction. Normalized peak intensities with a solid line Gaussian fit are shown in inset figures.

Fig. 5
Fig. 5

Multiphoton images of 6 μm beads fixed in agarose measured with 0.18 and 0.50 NA in (a) and (b), respectively. The horizontal scale bars are 100 μm .

Fig. 6
Fig. 6

(a),(b) SHG and (c),(d) reflection images of a rat-tail tendon, acquired with (b),(d) 0.50 and (a),(c) 0.18 NA. The scale bars are 100 μm .

Metrics