Abstract

We report the development of a miniaturized dual-optical-zone endoscope objective lens. The lens has two foci, with 0.18 and 0.50 NAs. We demonstrate multiphoton imaging with dual fields of view and resolutions using the new lens. A combination of multiphoton and single-photon microscopic imaging is also demonstrated.

© 2010 Optical Society of America

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2009 (2)

2008 (1)

2005 (1)

2003 (2)

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef] [PubMed]

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

1986 (1)

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[CrossRef] [PubMed]

Birk, D. E.

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[CrossRef] [PubMed]

Cobb, M. J.

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Fu, L.

Gan, X.

Gu, M.

Jung, J. C.

König, K.

Le Harzic, R.

Leng, Y.

Li, X. D.

Messerschmidt, B.

Riemann, I.

Schnitzer, M. J.

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Trelstd, R. L.

D. E. Birk and R. L. Trelstd, J. Cell Biol. 103, 231 (1986).
[CrossRef] [PubMed]

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[CrossRef]

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Weinigel, M.

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[CrossRef]

Wu, Y.

Xi, J.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, Nature Biotechnol. 21, 1369 (2003).
[CrossRef]

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Figures (6)

Fig. 1
Fig. 1

(a) Ray tracing diagram of the optical properties of the bifocal lens assembly. (b) Top view of a bifocal lens assembly in a lens holder. The center and peripheral optical zones are indicated by the image circular truncation of an underlying grid pattern.

Fig. 2
Fig. 2

Schematic diagram of the experiment setup for one-photon reflectance and multiphoton imaging.

Fig. 3
Fig. 3

Reflection images of a USAF target measured with NAs of (a) 0.18 and (b) 0.5. The insets in (a) and (b) are magnified images of the bars highlighted by the square. For all images, a 1 Hz frame rate was used.

Fig. 4
Fig. 4

Fluorescence intensity axial profile (in logarithmic scale) of both foci through the center of a fluorescence bead along the axial direction. Normalized peak intensities with a solid line Gaussian fit are shown in inset figures.

Fig. 5
Fig. 5

Multiphoton images of 6 μm beads fixed in agarose measured with 0.18 and 0.50 NA in (a) and (b), respectively. The horizontal scale bars are 100 μm .

Fig. 6
Fig. 6

(a),(b) SHG and (c),(d) reflection images of a rat-tail tendon, acquired with (b),(d) 0.50 and (a),(c) 0.18 NA. The scale bars are 100 μm .

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