Abstract

We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2π ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified.

© 2010 Optical Society of America

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2010

2009

2008

2007

2005

J. Müller, V. Kebbel, and W. Jüptner, Opt. Lasers Eng. 43, 739 (2005).
[CrossRef]

2003

2002

2000

1984

1947

A. T. Forrester, W. E. Parkins, and E. Gerjuoy, Phys. Rev. 72, 728 (1947).
[CrossRef]

Ampem-Lassen, E.

Barty, A.

Baxter, G.

Brady, D. J.

D. J. Brady, Optical Imaging and Spectroscopy (Wiley, 2009), pp. 268–272.

Brown, W. J.

Chalut, K. J.

Charrière, F.

Cheng, Y. Y.

Chilkoti, A.

Clark, R. L.

Colomb, T.

Cuche, E.

Dakoff, A.

De Nicola, S.

Depeursinge, C.

Desse, J. M.

Dragomir, N.

Emery, Y.

Ferraro, P.

Finan, J. D.

N. T. Shaked, J. D. Finan, F. Guilak, and A. Wax, J. Biomed. Opt. 15, 010505 (2010).
[CrossRef] [PubMed]

Finizio, A.

Forrester, A. T.

A. T. Forrester, W. E. Parkins, and E. Gerjuoy, Phys. Rev. 72, 728 (1947).
[CrossRef]

Gass, J.

Gerjuoy, E.

A. T. Forrester, W. E. Parkins, and E. Gerjuoy, Phys. Rev. 72, 728 (1947).
[CrossRef]

Ghiglia, D. C.

D. C. Ghiglia and M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, And Software (Wiley, 1998).

Grilli, S.

Guilak, F.

N. T. Shaked, J. D. Finan, F. Guilak, and A. Wax, J. Biomed. Opt. 15, 010505 (2010).
[CrossRef] [PubMed]

Hill, R. T.

Hucknall, A.

Huntington, S.

Jenness, N. J.

Jüptner, W.

J. Müller, V. Kebbel, and W. Jüptner, Opt. Lasers Eng. 43, 739 (2005).
[CrossRef]

Kebbel, V.

J. Müller, V. Kebbel, and W. Jüptner, Opt. Lasers Eng. 43, 739 (2005).
[CrossRef]

Kim, M.

Kühn, J.

Laporta, P.

Marquet, P.

Miccio, L.

Montfort, F.

Mounier, D.

Müller, J.

J. Müller, V. Kebbel, and W. Jüptner, Opt. Lasers Eng. 43, 739 (2005).
[CrossRef]

Nugent, K.

Osellame, R.

Parkins, W. E.

A. T. Forrester, W. E. Parkins, and E. Gerjuoy, Phys. Rev. 72, 728 (1947).
[CrossRef]

Paturzo, M.

Picart, P.

Pritt, M. D.

D. C. Ghiglia and M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, And Software (Wiley, 1998).

Rinehart, M. T.

Roberts, A.

Shaked, N. T.

N. T. Shaked, J. D. Finan, F. Guilak, and A. Wax, J. Biomed. Opt. 15, 010505 (2010).
[CrossRef] [PubMed]

N. T. Shaked, Y. Zhu, M. T. Rinehart, and A. Wax, Opt. Express 17, 15585 (2009).
[CrossRef] [PubMed]

Wax, A.

Wyant, J. C.

Zhu, Y.

Appl. Opt.

J. Biomed. Opt.

N. T. Shaked, J. D. Finan, F. Guilak, and A. Wax, J. Biomed. Opt. 15, 010505 (2010).
[CrossRef] [PubMed]

Opt. Express

Opt. Lasers Eng.

J. Müller, V. Kebbel, and W. Jüptner, Opt. Lasers Eng. 43, 739 (2005).
[CrossRef]

Opt. Lett.

Phys. Rev.

A. T. Forrester, W. E. Parkins, and E. Gerjuoy, Phys. Rev. 72, 728 (1947).
[CrossRef]

Other

D. J. Brady, Optical Imaging and Spectroscopy (Wiley, 2009), pp. 268–272.

D. C. Ghiglia and M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, And Software (Wiley, 1998).

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Figures (3)

Fig. 1
Fig. 1

Optical system based on a modified Mach–Zehnder interferometric microscope with two illumination lasers. Inset, pixel filter layout of a Bayer mosaic color camera.

Fig. 2
Fig. 2

SEM images of optical adhesive microstructures ( 2170 × magnification): (a) en face view with 15 μm lateral scale bar; (b) 45 ° tilt view with 15 μm vertical scale bar.

Fig. 3
Fig. 3

Microstructure OPD maps and profiles: (a) 532 nm OPD map after quality-map guided unwrapping, 15 μm lateral scale bar; (b) 532 nm OPD map after two- wavelength unwrapping, 15 μm lateral scale bar; (c) incorrect object height profile, from the dotted line in (a); (d) object height profile from two-wavelength unwrapping, from the dotted line in (b). The quantitative height measurements obtained from QPM agree with the SEM images in Fig. 2.

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