Abstract

Optoacoustic tomography can visualize optical contrast in tissues while capitalizing on the advantages of ultrasound, such as high spatial resolution and fast imaging capabilities. We report a novel multispectral optoacoustic tomography system for deep tissue small animal imaging. The previously undocumented capacity of whole-body optoacoustic tomography at a video rate has been demonstrated by visualizing mouse kidney perfusion using Indocyanine Green in vivo.

© 2010 Optical Society of America

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  1. V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
    [CrossRef]
  2. L. V. Wang, Med. Phys. 35, 5758 (2008).
    [CrossRef]
  3. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
    [CrossRef]
  4. D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
    [CrossRef] [PubMed]
  5. R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
    [CrossRef] [PubMed]
  6. X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
    [CrossRef]
  7. S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
    [CrossRef] [PubMed]
  8. B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
    [CrossRef] [PubMed]
  9. H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
    [CrossRef]
  10. J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
    [CrossRef] [PubMed]
  11. C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
    [CrossRef]
  12. B. A. J. Angelsen, Ultrasound Imaging (Emantec AS, 2000).
  13. A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
    [CrossRef] [PubMed]
  14. M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
    [PubMed]

2010 (1)

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

2009 (6)

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

2008 (1)

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

2007 (1)

2005 (1)

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

2004 (1)

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

2003 (1)

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

1976 (1)

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Aguirre, A.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Angelsen, B. A. J.

B. A. J. Angelsen, Ultrasound Imaging (Emantec AS, 2000).

Brecht, H. P.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Chen, Q.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Conjusteau, A.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ermilov, S. A.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Fronheiser, M.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Gamelin, J.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Guo, P. Y.

Huang, F.

Khamapirad, T.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Koster, R. W.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ku, G.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Kwant, G.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Lacewell, R.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Landsman, M. L. J.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Leonard, M. H.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Li, C.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

Ma, R.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

Maurudis, A.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Mehta, K.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Miller, T.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Mook, G. A.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Ntziachristos, V.

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Oraevsky, A. A.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Pang, Y. J.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Razansky, D.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Ripoll, J.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Rosenthal, A.

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

Stoica, G.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Su, R.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Tan, Y.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Taruttis, A.

Vinegoni, C.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Wang, L. H. V.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Wang, L. V.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

Wang, X. D.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Wang, Y.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Weissleder, R.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Xie, X. Y.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Xing, D.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Yin, B. Z.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Zeng, Y. G.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Zhu, Q.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Zijlstra, W. G.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

IEEE Trans. Med. Imaging (1)

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

J. Appl. Physiol. (1)

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

J. Biomed. Opt. (2)

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

J. Biomed. Opt. Lett. (1)

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

Med. Phys. (1)

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

Nat. Biotech. (2)

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Nat. Photon. (1)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Opt. Express (2)

Opt. Lett. (1)

Phys. Med. Biol. (1)

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Other (1)

B. A. J. Angelsen, Ultrasound Imaging (Emantec AS, 2000).

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic representation of the MSOT system. A curved array of wideband and cylindrically focused ultrasound transducers enables parallel data acquisition. Optical fibers are used to homogeneously illuminate the object. A special animal holder with a transparent plastic membrane is used for animal positioning. (b) Picture of the mouse during a scan, showing the position of the mouse and illumination with respect to the array of focused wideband ultrasound transducers.

Fig. 2
Fig. 2

(a) Stack of representative slices of a 3D dataset of the pelvis and kidney region of a female eight-week-old CD1 mouse. (b) Cross-sectional optoacoustic image before injection of the slice in which the kinetics of ICG was monitored. (c) Photograph of a cross-sectional cryoslice at a height corresponding to that shown in (b): 1, vena cava; 2, portal vein; 3, kidneys; 4, spinal cord; 5, backbone muscles; and 6, spleen.

Fig. 3
Fig. 3

(a) Cross-sectional optoacoustic images at different time points of the kidneys of a female CD1 mouse illuminated at 800 nm after having injected 300 nmol of ICG. (b) Superposition of the absorption difference and the single- wavelength image before injection.

Fig. 4
Fig. 4

(a) Superposition of a single wavelength image ( 890 nm ) and the unmixed component corresponding to the ICG signal. (b) Molar extinction coefficient of ICG [14], oxygenized and deoxygenized hemoglobin [4].

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