Abstract

Several recently developed particle-tracking and imaging methods have achieved three-dimensional sensitivity through the introduction of angled micromirrors into the observation volume of an optical microscope. We model the imaging response of such devices and show how the direct and reflected images of a fluorescent particle are affected. In particle-tracking applications, asymmetric image degradation manifests itself as systematic tracking errors. Based on our results, we identify strategies for reducing systematic errors to the 10nm level in practical applications.

Full Article  |  PDF Article
OSA Recommended Articles
Properties of coherence-gated wavefront sensing

Markus Rueckel and Winfried Denk
J. Opt. Soc. Am. A 24(11) 3517-3529 (2007)

Correcting field-dependent aberrations with nanoscale accuracy in three-dimensional single-molecule localization microscopy

Alex von Diezmann, Maurice Y. Lee, Matthew D. Lew, and W. E. Moerner
Optica 2(11) 985-993 (2015)

Comparative analysis of imaging configurations and objectives for Fourier microscopy

Jonathan A. Kurvits, Mingming Jiang, and Rashid Zia
J. Opt. Soc. Am. A 32(11) 2082-2092 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription