Abstract

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical–mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5kHz and two-dimensional B-scan images containing 800 A-lines at 6.25Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128s for an 800×800×200 data set and of the ear vasculature in 256s for an 800×1600×200 data set.

© 2010 Optical Society of America

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Supplementary Material (2)

» Media 1: MPG (4839 KB)     
» Media 2: MPG (3435 KB)     

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