Abstract

We describe methods and algorithms for rapid volumetric imaging of cortical vasculature with optical coherence tomography (OCT). By optimizing system design, scanning protocols, and algorithms for visualization of capillary flow, comprehensive imaging of the surface pial vasculature and capillary bed is performed in approximately 12s. By imaging during hypercapnia and comparing with simultaneous CCD imaging, the sources of contrast of OCT angiography are investigated.

© 2009 Optical Society of America

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References

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R. K. Wang and L. An, Opt. Express 17, 8926 (2009).
[CrossRef] [PubMed]

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[CrossRef] [PubMed]

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2007

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E. B. Hutchinson, B. Stefanovic, A. P. Koretsky, and A. C. Silva, Neuroimage 32, 520 (2006).
[CrossRef] [PubMed]

1998

1969

H. Schmid-Schoenbein and R. Wells, Science 165, 288 (1969).
[CrossRef] [PubMed]

Alsholm, P.

An, L.

Andersson-Engels, S.

Bouma, B. E.

B. J. Vakoc, G. J. Tearney, and B. E. Bouma, IEEE Trans. Med. Imaging 28, 814 (2009).
[CrossRef] [PubMed]

Cable, A.

Davis, A. M.

Fingler, J.

Fraser, S. E.

Gruber, A.

Hanson, S. R.

Hurst, S.

Hutchinson, E. B.

E. B. Hutchinson, B. Stefanovic, A. P. Koretsky, and A. C. Silva, Neuroimage 32, 520 (2006).
[CrossRef] [PubMed]

Izatt, J. A.

Jacques, S. L.

Jiang, J.

Karlsson, A.

Khurana, M.

Koretsky, A. P.

E. B. Hutchinson, B. Stefanovic, A. P. Koretsky, and A. C. Silva, Neuroimage 32, 520 (2006).
[CrossRef] [PubMed]

Leung, M. K.

Ma, Z.

Mariampillai, A.

Moriyama, E. H.

Munce, N. R.

Nilsson, A. M.

Schmid-Schoenbein, H.

H. Schmid-Schoenbein and R. Wells, Science 165, 288 (1969).
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Schwartz, D.

Silva, A. C.

E. B. Hutchinson, B. Stefanovic, A. P. Koretsky, and A. C. Silva, Neuroimage 32, 520 (2006).
[CrossRef] [PubMed]

Standish, B. A.

Stefanovic, B.

E. B. Hutchinson, B. Stefanovic, A. P. Koretsky, and A. C. Silva, Neuroimage 32, 520 (2006).
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Tao, Y. K.

Tearney, G. J.

B. J. Vakoc, G. J. Tearney, and B. E. Bouma, IEEE Trans. Med. Imaging 28, 814 (2009).
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Vakoc, B. J.

B. J. Vakoc, G. J. Tearney, and B. E. Bouma, IEEE Trans. Med. Imaging 28, 814 (2009).
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Vitkin, I. A.

Wang, R. K.

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Wilson, B. C.

Yang, C.

Yang, V. X.

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Figures (4)

Fig. 1
Fig. 1

(A) OCT system and microscope schematic. (B) Three-dimensional scan protocol for performing OCT angiography that samples each transverse location twice. (C),(D) Examples of cross-sectional OCT angiograms generated by phase-insensitive and phase-sensitive methods described in the text.

Fig. 2
Fig. 2

(A) CCD image and (B) wide-field OCT angiogram of cranial window with (C) enlargement showing microvasculature. (D) 3D oblique slices and cross-sectional image (inset) showing distinct backscattering pattern due to shear-induced orientation of RBCs.

Fig. 3
Fig. 3

(A) CCD image and (B) OCT angiogram of cortical vasculature during normocapnia. (C) Changes in vessel tone in representative artery A and vein V during 7.5% hypercapnia, shown as a line along the time axis. (D) Transient perfusion of arterial anastomosis during hypercapnia visualized by CCD imaging (above) and confirmed by OCT (below).

Fig. 4
Fig. 4

(A), (C), Media 1: OCT imaging of the capillary bed during normocapnia. (B), (D), Media 2: hypercapnia, demonstrating flow redistribution (arrows).

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