Abstract

In in-line holographic microscopy a pinhole illuminates an object and a CCD-detector directly records the hologram in a pixel-pitch-dependent distance. A rapidly calculating exact reconstruction technique using a reorganized hologram with a low number of pixels, the tile superposition technique, is presented. The algorithm is applied on imaging of a $2\mu \mathrm{m}$ bead cluster, and it is compared with other reconstruction techniques. The high-contrast image corresponds to an NA of 0.7. A full $4\text{\hspace{0.17em} megapixel}$ reconstruction with a resolution approaching the diffraction limit is possible in less than a minute. The technique is a base for high-resolution wide-field imaging by multispot illumination.

© 2009 Optical Society of America

Fast exact scalar propagation for an in-line holographic microscopy on the diffraction limit

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