The most common and lethal birth defects affect the cardiovascular (CV) system. The mouse is a superior model for identifying and understanding mammalian CV birth defects, but there is a great need for tools that can detect early and subtle deficiencies in cardiac function in mouse embryos. We combined swept source optical coherence tomography (SS-OCT) with live mouse embryo culture protocols to generate structural two-dimensional and three-dimensional imaging and hemodynamic measurements in a live embryo just a few hours after the beginning of a heartbeat. Our data show that individual circulating blood cells can be visualized with structural SS-OCT, and using Doppler SS-OCT the velocity of single moving blood cells were measured during different phases of the heartbeat cycle. These results demonstrate that Doppler SS-OCT is an extremely useful tool for structural and hemodynamic analysis at the earliest stages of mammalian blood circulation.
© 2009 Optical Society of AmericaFull Article | PDF Article
Nicusor V. Iftimia, Daniel X. Hammer, R. Daniel Ferguson, Mircea Mujat, Danthu Vu, and Anthony A. Ferrante
Opt. Express 16(18) 13624-13636 (2008)
A. M. Davis, F. G. Rothenberg, N. Shepherd, and J. A. Izatt
J. Opt. Soc. Am. A 25(12) 3134-3143 (2008)
Zhenhe Ma, Aiping Liu, Xin Yin, Aaron Troyer, Kent Thornburg, Ruikang K. Wang, and Sandra Rugonyi
Biomed. Opt. Express 1(3) 798-811 (2010)