Abstract

We report a scheme for 2D standing-wave total-internal-reflection fluorescence microscopy. Standing-wave patterns are generated by two interfering beams coupled through the objective lens. Period, angular orientation, and phase of standing waves are controlled entirely by acousto-optic deflectors. The lateral resolution improvement of 100nm is combined with an axial selectivity of <100nm by utilizing an evanescent standing-wave pattern. This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass–water interface.

© 2009 Optical Society of America

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