Abstract

We present a normalized Born approach for fluorescence optical projection tomography that takes into account tissue absorption properties. This approach can be particularly useful to study fluorochrome distribution within tissue. We use the algorithm to three-dimensionally reconstruct and characterize a fluorescein isothiocyanate containing absorptive phantom and an infarcted mouse heart previously injected with a fluorescent molecular probe.

© 2009 Optical Society of America

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References

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  1. J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
    [CrossRef] [PubMed]
  2. K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
    [CrossRef] [PubMed]
  3. M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
    [CrossRef]
  4. V. Ntziachristos and R. Weissleder, Opt. Lett. 26, 893 (2001).
    [CrossRef]

2007

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

2006

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

2002

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

2001

Ahlgren, U.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Avondo, J.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Baldock, R.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Bangham, A.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Bentley, R.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Blot, L.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Coen, E.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Davidson, D.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Dewhirst, M.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Guo, P. Y.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Hecksher-Sorensen, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Hill, B.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Lee, K.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Morrison, H.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Ntziachristos, V.

Oldham, M.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Oliver, T.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Perry, P.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Ross, A.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Sakhalkar, H.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Sharpe, J.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Stark, M.

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Vujaskovic, Z.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Wang, Y. M.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Weissleder, R.

J. Biomed. Opt.

M. Oldham, H. Sakhalkar, Y. M. Wang, P. Y. Guo, T. Oliver, R. Bentley, Z. Vujaskovic, and M. Dewhirst, J. Biomed. Opt. 12, 1 (2007).
[CrossRef]

Opt. Lett.

Plant Cell

K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen, Plant Cell 18, 2145 (2006).
[CrossRef] [PubMed]

Science

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) Experimental setup. LS, light source; S, shutter; BE, beam expander; BPF, filters; S, sample; IS, imaging system. (b) Schematic of the gel agarose phantom. The phantom consists of two separate cylinders fused together along their vertical axis, with the bottom one containing India ink ( 10 ppm ) . A hole is bored into the final fused cylinder along its vertical axis and filled with a 1 μ M concentration of FITC dextran. (c) Phantom’s fluorescence and (d) absorption projections.

Fig. 2
Fig. 2

Reconstructions at two different positions (I and II) along the phantom vertical axis. (a) Absorption (b) plain fluorescence, and (c) normalized Born reconstructions. (d) and (e) Fluorescence intensity plots along the reconstructions’ center at level I and II are shown in dashed gray and continuous black, respectively. (d) Without and (e) with normalization correction.

Fig. 3
Fig. 3

Reconstructions of a mouse heart that had previously sustained a controlled infarct and was injected with Prosense-680. (a) Histology, (b) absorption reconstruction, (c) plain fluorescence reconstruction, and (d) normalized Born fluorescence reconstruction. The distribution of the fluorescent probe in (d) has been confirmed by correlative immunohistochemistry.

Equations (4)

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U B ( x s , x d ) = U fl ( x s , x d ) U ex ( x s , x d ) .
G ex ( x s , x ) = e x s x μ a ( x ) d x .
U fl ( x s , x d ) = D x s x d G ex ( x s , x ) A ( x ) G fl ( x , x d ) d x ,
U B ( x s , x d ) = α G ex ( x s , x d ) x s x d G ex ( x s , x ) A ( x ) G ex ( x , x d ) d x ,

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