Abstract

Low-coherence spectroscopy (LCS) is a spectroscopic method that allows for quantitative and localized assessment of absorption spectra by combining reflection spectroscopy with low-coherence interferometry. We describe absorption coefficient (μa) measurements by LCS in tissue simulating phantoms with varying scattering and absorbing properties. We used LCS in the 455680nm wavelength range with a spectral resolution of 8nm to obtain μa spectra with ±0.5mm1 accuracy. We conclude that LCS is a promising technique for the in vivo determination of tissue chromophore concentrations.

© 2009 Optical Society of America

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2009 (1)

2008 (1)

2007 (1)

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

2004 (3)

2002 (1)

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

2000 (2)

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

P. Rolfe, Annu. Rev. Biomed. Eng. 2, 715 (2000).
[CrossRef]

1998 (1)

1997 (1)

1988 (1)

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
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[CrossRef]

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Bizheva, K. K.

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D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

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A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
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Hermann, B.

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Marks, D. L.

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A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

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[CrossRef]

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[CrossRef] [PubMed]

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P. Rolfe, Annu. Rev. Biomed. Eng. 2, 715 (2000).
[CrossRef]

Sattman, H.

Schmetterer, L.

Siegel, A. M.

Steenbergen, W.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

Sterenborg, H. J. C. M.

Tromberg, B. J.

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

Unterhuber, A.

van der Zee, P.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

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A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

van Leeuwen, T. G.

D. J. Faber and T. G. van Leeuwen, Opt. Lett. 34, 1435 (2009).
[CrossRef] [PubMed]

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

Varghese, B.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

Welch, A. J.

A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

Wray, S.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Wyatt, J.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Xu, C.

Annu. Rev. Biomed. Eng. (1)

P. Rolfe, Annu. Rev. Biomed. Eng. 2, 715 (2000).
[CrossRef]

Appl. Opt. (1)

Appl. Phys. Lett. (1)

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

J. Biomed. Opt. (1)

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (3)

Phys. Med. Biol. (1)

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Rev. Sci. Instrum. (1)

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

Other (1)

A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

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Figures (3)

Fig. 1
Fig. 1

LCS system (upper part) and a schematic overview of the signal processing (lower part). LPF, low-pass filter; BS, beam splitter; NDF, neutral density filter; R, piezo-driven reference mirror; Δ R , scanning range of R; MM, multimode graded index detection fiber; FT, Fourier transform; PS, polystyrene spheres; l , geometrical optical path length in the sample; λ, wavelength; S, power spectrum; μ t μ a , attenuation/absorption coefficient.

Fig. 2
Fig. 2

Attenuation spectra for three concentrations of polystyrene spheres (PS) with and without dye. Error bars represent the 95% c.i. of the fitted values. The lines through the data points are drawn as a guide to the eye.

Fig. 3
Fig. 3

Absorption spectra for three samples with varying concentrations of PS but constant concentration of absorbing dye. Error bars represent the 95% c.i. of the fitted values.

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