Abstract

Low-coherence spectroscopy (LCS) is a spectroscopic method that allows for quantitative and localized assessment of absorption spectra by combining reflection spectroscopy with low-coherence interferometry. We describe absorption coefficient (μa) measurements by LCS in tissue simulating phantoms with varying scattering and absorbing properties. We used LCS in the 455680nm wavelength range with a spectral resolution of 8nm to obtain μa spectra with ±0.5mm1 accuracy. We conclude that LCS is a promising technique for the in vivo determination of tissue chromophore concentrations.

© 2009 Optical Society of America

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B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

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A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

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T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

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[CrossRef]

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D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
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Bizheva, K.

Bizheva, K. K.

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Boccara, C.

Boppart, S. A.

Burgers, S. A.

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D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
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de Mul, F. F. M.

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
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D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
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Do, M. N.

Drexler, W.

Dubois, A.

Faber, D. J.

Fercher, A. F.

Fishkin, J. B.

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
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Foster, T. H.

Hermann, B.

Hull, E. L.

Marks, D. L.

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Nichols, M. G.

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A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
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Pham, T. H.

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
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Povazay, B.

Rajan, V.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

Rolfe, P.

P. Rolfe, Annu. Rev. Biomed. Eng. 2, 715 (2000).
[CrossRef]

Sattman, H.

Schmetterer, L.

Siegel, A. M.

Steenbergen, W.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

Sterenborg, H. J. C. M.

Tromberg, B. J.

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

Unterhuber, A.

van der Zee, P.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

van Gemert, M. J. C.

A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

van Leeuwen, T. G.

D. J. Faber and T. G. van Leeuwen, Opt. Lett. 34, 1435 (2009).
[CrossRef] [PubMed]

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

Varghese, B.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

Welch, A. J.

A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

Wray, S.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Wyatt, J.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Xu, C.

Annu. Rev. Biomed. Eng.

P. Rolfe, Annu. Rev. Biomed. Eng. 2, 715 (2000).
[CrossRef]

Appl. Opt.

Appl. Phys. Lett.

A. L. Petoukhova, W. Steenbergen, T. G. van Leeuwen, and F. F. M. de Mul, Appl. Phys. Lett. 81, 595 (2002).
[CrossRef]

J. Biomed. Opt.

B. Varghese, V. Rajan, T. G. van Leeuwen, and W. Steenbergen, J. Biomed. Opt. 12, 024020 (2007).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Phys. Med. Biol.

D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, Phys. Med. Biol. 33, 1433 (1988).
[CrossRef] [PubMed]

Rev. Sci. Instrum.

T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, Rev. Sci. Instrum. 71, 2500 (2000).
[CrossRef]

Other

A. J. Welch and M. J. C. van Gemert, Optical-Thermal Response of Laser-Irradiated Tissue (Plenum, 1995).

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Figures (3)

Fig. 1
Fig. 1

LCS system (upper part) and a schematic overview of the signal processing (lower part). LPF, low-pass filter; BS, beam splitter; NDF, neutral density filter; R, piezo-driven reference mirror; Δ R , scanning range of R; MM, multimode graded index detection fiber; FT, Fourier transform; PS, polystyrene spheres; l , geometrical optical path length in the sample; λ, wavelength; S, power spectrum; μ t μ a , attenuation/absorption coefficient.

Fig. 2
Fig. 2

Attenuation spectra for three concentrations of polystyrene spheres (PS) with and without dye. Error bars represent the 95% c.i. of the fitted values. The lines through the data points are drawn as a guide to the eye.

Fig. 3
Fig. 3

Absorption spectra for three samples with varying concentrations of PS but constant concentration of absorbing dye. Error bars represent the 95% c.i. of the fitted values.

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