Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

© 2009 Optical Society of America

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2008 (2)

2007 (2)

M. J. Booth, Philos. Trans. R. Soc. London, Ser. A 365, 2829 (2007).

D. Débarre, M. J. Booth, and T. Wilson, Opt. Express 15, 8176 (2007).
[Crossref] [PubMed]

2004 (2)

2003 (1)

1999 (1)

Beaurepaire, E.

Booth, M. J.

Botcherby, E. J.

Chen, S. U.

Chen, S. Y.

Chu, S. W.

Débarre, D.

Farge, E.

Hsieh, C. S.

Lee, Y. W.

Lin, C. Y.

Liu, T. M.

Moulia, B.

Schanne-Klein, M. C.

Schwertner, M.

Silberberg, Y.

Sun, C. K.

Supatto, W.

Tsai, H. J.

Tsai, T. H.

Wilson, T.

Yang, Y. S.

Yelin, D.

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Figures (4)

Fig. 1
Fig. 1

Schematic of the microscope. L x , lens; BS x , beam splitter; M x , mirror; DM, deformable mirror; O, objective; C, condenser; D x , detector.

Fig. 2
Fig. 2

Correction of system aberrations. (a) THG axial response at a glass/air interface before (blue dashed line) and after correction. (b) THG signal (blue dashed line) and incremental retrieved phase aberration for subsequently applied correction cycles.

Fig. 3
Fig. 3

(a) THG images of a 5.5  day old live mouse embryo with correction of only system aberrations (left) and after additional correction of specimen-induced aberrations (right). The dashed lines show where the x y and x z planes intersect. The corresponding correction phase functions are also shown. (b) Intensity profiles along the solid lines A–B and C–D as drawn in (a).

Fig. 4
Fig. 4

Equivalence of correction using SHG and THG signals. (a) Overview of the 2.5  day old embryo showing SHG (solid red) and THG. (b) Sections used for the SHG- and THG-based corrections. The sections are located in close vicinity. Upper image pair, before correction; lower image pair, after correction. (c) Zernike coefficients and phase functions retrieved by both correction procedures.