Abstract

We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2(2N+1) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.

© 2009 Optical Society of America

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Supplementary Material (4)

» Media 1: AVI (13564 KB)     
» Media 2: AVI (4159 KB)     
» Media 3: AVI (9838 KB)     
» Media 4: AVI (3950 KB)     

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