Abstract

We present multiphoton microendoscopy with a rotational probe and a 1μm fiber-based femtosecond laser. The rotational probe is based on a double-clad photonic crystal fiber, a gradient index lens, a microprism, and a rotational microelectronicmechanical system (MEMS) motor. The MEMS motor has a diameter of 2.2mm and can provide 360° full-view rotation. The fiber laser provides ultrashort pulses with a central wavelength at 1.034μm and a repetition rate of 50MHz. Second-harmonic-generation images of rat-tail tendon and fish scale are demonstrated with the rotational probe-based multiphoton system.

© 2009 Optical Society of America

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2009

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

2008

2007

2006

2005

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

L. Fu, X. Gan, and M. Gu, Opt. Express 13, 5528 (2005).
[CrossRef] [PubMed]

2004

2003

Ahn, Y.

Baker, J. R.

Baldacchini, T.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Balu, M.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Bouwmans, G.

Carter, J.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Chen, Z.

Cheung, E. L. M.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Cocker, E. D.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Denk, W.

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

Engelbrecht1, C. J.

Flusberg, B. A.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Fu, L.

Gan, X.

Göbel, W.

Gu, M.

Helmchen, F.

Ilday, F. Ö.

Johnston, R. S.

Jung, J. C.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef] [PubMed]

Jung, W.

Kerr, J. N. D.

Knight, J. C.

Krasieva, T. B.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

W. Jung, S. Tang, D. T. McCormic, T. Xie, Y. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, Opt. Lett. 33, 1324 (2008).
[CrossRef] [PubMed]

Li, X.

Lim, H.

MacDonald, D. J.

McCormic, D. T.

Myaing, M. T.

Nimmerjahn, A. L.

Norris, T. B.

Piyawattanametha, W.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Russell, P. St. J.

Schnitzer, M. J.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef] [PubMed]

Seibel, E. J.

Su, J.

Tang, S.

Thomas, T.

Tomov, I. V.

Tromberg, B. J.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

W. Jung, S. Tang, D. T. McCormic, T. Xie, Y. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, Opt. Lett. 33, 1324 (2008).
[CrossRef] [PubMed]

B. J. Tromberg, in Handbook of Biomedical Nonlinear Optical Microscopy, B.R.Masters and P.T. C.So, eds. (Oxford U. Press, 2008), pp. 707-715.

Wadsworth, W. J.

Wise, F. W.

Xie, T.

Ye, J. Y.

Yu, L.

Zadoyan, R.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Zhang, J.

J. Biomed. Opt.

M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, J. Biomed. Opt. 14, 010508-1 (2009).
[CrossRef]

Nat. Med.

F. Helmchen, W. Denk, Nat. Med. 2, 932 (2005).
[CrossRef]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Med. 2, 941 (2005).
[CrossRef]

Opt. Express

Opt. Lett.

Other

B. J. Tromberg, in Handbook of Biomedical Nonlinear Optical Microscopy, B.R.Masters and P.T. C.So, eds. (Oxford U. Press, 2008), pp. 707-715.

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Figures (4)

Fig. 1
Fig. 1

Diagram of the multiphoton imaging system. A, fiber laser oscillator; B, fiber amplifier; C, grating pair compressor; D, the microprobe, data acquisition, and processing system. WDM, wavelength-division multiplexer; PCF, photonic crystal fiber; HWP, half-wave plate; PMT, photomultiplier tube; AMP, amplifier.

Fig. 2
Fig. 2

Double-logarithmic plot of the SHG intensity from KDP crystal power as a function of incident power.

Fig. 3
Fig. 3

Diagram of the microrotational probe. The probe is based on a DCPCF, a GRIN lens, a microprism, and a rotational MEMS motor. (b) Photograph of the microprism glued to a MEMS motor together with a United States dime coin.

Fig. 4
Fig. 4

SHG images of (a) a rat tail tendon and (b) a fish scale. Scale bar is 25 μ m .

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