Abstract

We propose to achieve superresolution in far-field fluorescence imaging by use of silica nanoparticles codoped with Cy3 and Cy5 molecules at a controllable ratio. Owing to the short distances between the dye molecules confined in the nanoparticles, Förster resonant energy transfer can efficiently occur between the Cy3 and Cy5 molecules. Therefore, the Cy5 molecules can quench the fluorescence emission from the Cy3 molecules in the outer region of focal spot of the excitation beam when the excitation intensity is properly chosen, thereby enhancing the resolution of imaging.

© 2009 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. S. W. Hell, Science 316, 1153 (2007).
    [CrossRef] [PubMed]
  2. M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
    [CrossRef] [PubMed]
  3. M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
    [CrossRef] [PubMed]
  4. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
    [CrossRef]
  5. M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005).
    [CrossRef] [PubMed]
  6. M. C. Lang, J. Engelhardt, and S. W. Hell, Opt. Lett. 32, 259 (2007).
    [CrossRef] [PubMed]
  7. J. Chen and K. Midorikawa, Opt. Lett. 29, 1354 (2004).
    [CrossRef] [PubMed]
  8. L. Wang and W. Tan, Nano Lett. 6, 84 (2006).
    [CrossRef] [PubMed]
  9. A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
    [CrossRef]
  10. A. Schönle and S. W. Hell, Eur. Phys. J. D 6, 283 (1999).
    [CrossRef]

2007 (2)

2006 (3)

L. Wang and W. Tan, Nano Lett. 6, 84 (2006).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

2005 (1)

M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005).
[CrossRef] [PubMed]

2004 (1)

2002 (1)

M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
[CrossRef] [PubMed]

1999 (2)

A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
[CrossRef]

A. Schönle and S. W. Hell, Eur. Phys. J. D 6, 283 (1999).
[CrossRef]

Bates, M.

M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
[CrossRef] [PubMed]

Betzig, E.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Chen, J.

Davidson, M. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Dyba, M.

M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
[CrossRef] [PubMed]

Engelhardt, J.

Gustafsson, M. G. L.

M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005).
[CrossRef] [PubMed]

Hänninen, P. E.

A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
[CrossRef]

Hell, S. W.

M. C. Lang, J. Engelhardt, and S. W. Hell, Opt. Lett. 32, 259 (2007).
[CrossRef] [PubMed]

S. W. Hell, Science 316, 1153 (2007).
[CrossRef] [PubMed]

M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
[CrossRef] [PubMed]

A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
[CrossRef]

A. Schönle and S. W. Hell, Eur. Phys. J. D 6, 283 (1999).
[CrossRef]

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Lang, M. C.

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Midorikawa, K.

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Rust, M. J.

M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
[CrossRef] [PubMed]

Schönle, A.

A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
[CrossRef]

A. Schönle and S. W. Hell, Eur. Phys. J. D 6, 283 (1999).
[CrossRef]

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Tan, W.

L. Wang and W. Tan, Nano Lett. 6, 84 (2006).
[CrossRef] [PubMed]

Wang, L.

L. Wang and W. Tan, Nano Lett. 6, 84 (2006).
[CrossRef] [PubMed]

Zhuang, X. W.

M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
[CrossRef] [PubMed]

Ann. Phys. (1)

A. Schönle, P. E. Hänninen, and S. W. Hell, Ann. Phys. 8, 115 (1999).
[CrossRef]

Eur. Phys. J. D (1)

A. Schönle and S. W. Hell, Eur. Phys. J. D 6, 283 (1999).
[CrossRef]

Nano Lett. (1)

L. Wang and W. Tan, Nano Lett. 6, 84 (2006).
[CrossRef] [PubMed]

Nat. Methods (1)

M. J. Rust, M. Bates, and X. W. Zhuang, Nat. Methods 3, 793 (2006).
[CrossRef] [PubMed]

Opt. Lett. (2)

Phys. Rev. Lett. (1)

M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
[CrossRef] [PubMed]

Proc. Natl. Acad. Sci. USA (1)

M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005).
[CrossRef] [PubMed]

Science (2)

S. W. Hell, Science 316, 1153 (2007).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, Science 313, 1462 (2006).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (2)

Fig. 1
Fig. 1

(a) Schematic of dynamics showing fluorescence excitation of donor and resonant energy transfer between donor and acceptor. Inset, illustration of a dual-dye-doped nanoparticle. (b) Normalized fluorescence intensities as functions of the excitation intensity.

Fig. 2
Fig. 2

PSFs of a conventional 3D scanning fluorescence microscope using (a) Cy3 dye molecules and (b) dual-dye-doped nanoparticles as fluorescent labels.

Equations (7)

Equations on this page are rendered with MathJax. Learn more.

k F = ( 1 τ D ) [ R 0 r ] 6 ,
{ d n D E d t = n D G I E σ E n D E ( k D + k F ) d n A E d t = n D E k F n A E k A } , if n A G n D E ,
{ d n D E d t = n D G I E σ E n D E k D n A G k F d n A E d t = n A G k F n A E k A } , if n A G n D E ,
n D G + n D E = N D ,
n A G + n A E = N A ,
{ n D E = N D I E σ E ( I E σ E + k D + k F ) , if n A G = N A ( k F k A ) n D E n D E , n D E = [ N D I E σ E ( N A k A k F ) ( k A + k F ) ] ( I E σ E + k D ) , if n A G = N A k A ( k A + k F ) n D E . }
{ n D E N D I E σ E k F , if I E ( k A N A ) ( σ E N D ) , n D E [ N D I E σ E N A k A ] ( I E σ E + k D ) , if I E ( k A N A ) ( σ E N D ) . }

Metrics