Abstract

We present a combined optical coherence tomography (OCT) and line-scanning fluorescence laminar optical tomography (FLOT) system. This hybrid system enables coregistered structural and molecular imaging in 3D with 10100μm resolution and millimeter-scale imaging depth. Experimental results on a capillary phantom with fluorescence dye Cy5.5 using an OCT/FLOT imaging system have been demonstrated.

© 2009 Optical Society of America

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  11. A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988).

2007 (3)

2006 (2)

2004 (2)

2003 (1)

2000 (1)

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Aguirre, A. D.

Aoki, G.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Barton, J. K.

Bastacky, S.

Bernus, O.

Bigelow, C. E.

Boas, D.

Boas, D. A.

Bouchard, M. B.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Chen, Y.

Dale, A. M.

de Boer, J. F.

DiMarzio, C. A.

Du, C. W.

Dunn, A.

Dunn, A. K.

Dwyer, P. J.

Endo, T.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Ferguson, R. D.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

Y. Chen, S. W. Huang, A. D. Aguirre, and J. G. Fujimoto, Opt. Lett. 32, 1971 (2007).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hammer, D. X.

Hariri, L. P.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hillman, E. M. C.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Huang, S. W.

Iftimia, N. V.

Itoh, M.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Kak, A. C.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988).

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Makita, S.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Meyers, S.

Pan, Y. T.

Pease, E.

Pertsov, A.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Rajadhyaksha, M.

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Slaney, M.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988).

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Tumlinson, A. R.

Ustun, T.

Utzinger, U.

Xie, T. Q.

Yasuno, Y.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Yatagai, T.

Y. Yasuno, T. Endo, S. Makita, G. Aoki, M. Itoh, and T. Yatagai, J. Biomed. Opt. 11, 014014 (2006).
[CrossRef] [PubMed]

Zeidel, M. L.

Supplementary Material (1)

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Figures (3)

Fig. 1
Fig. 1

Schematic of the combined OCT and line-scan FLOT system.

Fig. 2
Fig. 2

(A) Monte Carlo simulated sensitivity distributions for line source and detector with separation d = 1.5 mm. Log scale from 4 to 0. (B) Intensity profiles for point object scanned through the Y axis (solid curve, depth 0 mm ; dashed curve, 0.8 mm ). (C)–(D) Raw FLOT measurement (XT) for different source-detector separations (left, Y = 0.5 mm; right, Y = 1.5 mm) for object depth (C) ~0 mm and (D) ~0.8 mm.

Fig. 3
Fig. 3

Coregistration of OCT and line-scan FLOT of a capillary tube filled with fluorescence dye. The direction of the tube is denoted by the dotted line in the CCD field-of-view in Fig. 1 (A) 3D OCT imaging showing three representative slices (the two dots above the capillary tube are due to impurities in the liquid phantom). (B) Left, OCT image (slice #1) of the capillary tube (arrow); right, FLOT image reconstruction of the fluorescence object. (C) OCT and FLOT images of different position (slice #2). (D) OCT and FLOT image (slice #3) reveal the curvature of the capillary tube (two identical dotted lines serve as the reference slopes). (E) 3D isosurface of FLOT image shows good colocalization with OCT (Media 1).

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