Abstract

We report experimental evidence and correction of defocus in full-field optical coherence tomography of biological samples owing to mismatch of the refractive index of biological tissues and water. Via a metric based on the image quality, we demonstrate that we are able to compensate this index-induced defocus and to recover a sharp image in depth.

© 2009 Optical Society of America

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References

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    [CrossRef] [PubMed]

2007 (1)

2006 (1)

M. Rueckel, J. A. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 46, 17137 (2006).
[CrossRef] [PubMed]

2004 (1)

2003 (1)

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn, Proc. SPIE 4956, 65 (2003).
[CrossRef]

2002 (2)

1996 (2)

1995 (2)

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, Opt. Lett. 20, 2258 (1995).
[CrossRef] [PubMed]

Artal, P.

Beaurepaire, E.

Boccara, A. C.

Booth, M. J.

Boppart, S. A.

G. J. Tearney, B. E. Bouma, S. A. Boppart, B. Golubovic, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 21, 1408 (1996).
[CrossRef] [PubMed]

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

Bouma, B. E.

Brezinski, M. E.

G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, Opt. Lett. 20, 2258 (1995).
[CrossRef] [PubMed]

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

Debarre, D.

Denk, W.

M. Rueckel, J. A. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 46, 17137 (2006).
[CrossRef] [PubMed]

Drexler, W.

Dubois, A.

Fercher, A. F.

Fernandez, E. J.

Fujimoto, J. G.

Golubovic, B.

Hee, M. R.

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, Opt. Lett. 20, 2258 (1995).
[CrossRef] [PubMed]

Hermann, B.

Jonnal, R. S.

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn, Proc. SPIE 4956, 65 (2003).
[CrossRef]

Mack-Bucher, J. A.

M. Rueckel, J. A. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 46, 17137 (2006).
[CrossRef] [PubMed]

Miller, D. T.

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn, Proc. SPIE 4956, 65 (2003).
[CrossRef]

Prieto, P. M.

Qu, J.

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn, Proc. SPIE 4956, 65 (2003).
[CrossRef]

Rueckel, M.

M. Rueckel, J. A. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 46, 17137 (2006).
[CrossRef] [PubMed]

Sattmann, H.

Southern, J. F.

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, Opt. Lett. 20, 2258 (1995).
[CrossRef] [PubMed]

Swanson, E. A.

G. J. Tearney, B. E. Bouma, S. A. Boppart, B. Golubovic, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 21, 1408 (1996).
[CrossRef] [PubMed]

J. G. Fujimoto, M. E. Brezinski, G. J. Tearney, S. A. Boppart, B. E. Bouma, M. R. Hee, J. F. Southern, and E. A. Swanson, Nat. Med. 1, 970 (1995).
[CrossRef] [PubMed]

Tearney, G. J.

Thorn, K.

D. T. Miller, J. Qu, R. S. Jonnal, and K. Thorn, Proc. SPIE 4956, 65 (2003).
[CrossRef]

Unterhubner, A.

Vabre, L.

Wilson, T.

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Figures (3)

Fig. 1
Fig. 1

Principle of the defocusing phenomenon. In a tissue with index n higher than water, the coherence plane (corresponding to zero-path difference) is shifted upward, while the focus is moved downward.

Fig. 2
Fig. 2

FF-OCT image for four different values of added defocus δ. The sample is a sentinel lymph node, and the width of the field of view is 500 μ m . The optimal image (for δ = + 30 μ m ) is both brighter and sharper.

Fig. 3
Fig. 3

Polar Fourier spectrum as a function of the amount of defocus introduced. Spectra are normalized to the mean image intensity. Inset, the FFT of the optimal image. (b) Figure of merit as a function of defocus δ z and Lorentzian fit to obtain the optimal focus position.

Equations (1)

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δ f ( z ) = 2 n ( F ( z ) + Π ( z ) ) = 2 n z ( n n n n ) = 2 z n 2 n 2 n .

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