Abstract

Capillaries, the smallest blood vessels, are the distal end of the vasculature where oxygen and nutrients are exchanged between blood and tissue. Hence, noninvasive imaging of capillaries and function in vivo has long been desired as a window to studying fundamental physiology, such as neurovascular coupling. Existing imaging modalities cannot provide the required sensitivity and spatial resolution. We present in vivo imaging of the microvasculature including single capillaries in mice using optical-resolution photoacoustic microscopy (OR-PAM) developed in our laboratory. OR-PAM provides a lateral resolution of 5μm and an imaging depth >0.7mm.

© 2008 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
    [CrossRef] [PubMed]
  2. A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.
  3. K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
    [CrossRef] [PubMed]
  4. H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
    [CrossRef]
  5. A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).
  6. S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
    [CrossRef] [PubMed]
  7. D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
    [CrossRef] [PubMed]
  8. V. P. Zharov, E. I. Galanzha, E. V. Shashkov, N. G. Khlebtsov, and V. V. Tuchin, Opt. Lett. 31, 3623 (2006).
    [CrossRef] [PubMed]
  9. Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

2007

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

2006

2005

2001

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Arbeit, J. M.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Basu, S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Bliss, V. S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Dasgupta, P. S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Dovrak, H. F.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Eckelhoefer, I. A.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Elson, D. A.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Galanzha, E. I.

Ginzinger, D. G.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Guyton, A. C.

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

Hall, J. E.

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

Huang, L. E.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Johnson, R. S.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Karabutov, A. A.

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Khlebtsov, N. G.

Manseau, E. J.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Maslov, K.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef] [PubMed]

McDonald, D. M.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Mukhopadhyay, D.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Nagy, J. A.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Oraevsky, A. A.

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Pal, S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Shashkov, E. V.

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef] [PubMed]

Thurston, G.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Tuchin, V. V.

Vasile, E.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Wang, L. V.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef] [PubMed]

Zhang, H. F.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Zharov, V. P.

Genes Dev.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
[CrossRef] [PubMed]

Nat. Biotechnol.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Nat. Med.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
[CrossRef] [PubMed]

Nat. Protocols

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
[CrossRef]

Opt. Lett.

Other

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

Schematic of the OR-PAM system. a, Condenser lens; b, pinhole; c, microscope objective; d, ultrasonic transducer; e, correcting lens; f, isosceles prism; g, acoustic lens; h, silicon oil.

Fig. 2
Fig. 2

Imaging depth and lateral resolution of the OR-PAM system. A, MAP image of two horse hairs placed above and below a piece of rat skin; B, B-scan image at the location marked by the dashed line in A; C, MAP image of a USAF resolution test target group 6; D, MAP image of a 6 μ m diameter carbon fiber; E, Cross-sectional profile of a 6 μ m diameter carbon fiber under a 0.2 mm thick nude mouse ear.

Fig. 3
Fig. 3

Microvasculature in a nude mouse ear. A, In vivo OR-PAM image; B, 3D pseudocolor visualization of the vasculature; C, photograph taken with transmission optical microscopy; CL, capillary, and SG, sebaceous gland.

Metrics