Abstract

Subnanosecond supercontinuum (SC) has been generated by a 1064nm microchip laser combined with a photonic crystal fiber. The ultrabroadband (>2000cm1) SC has facilitated multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy in the spectral range from 1000 to 3000cm1 with lateral and depth spatial resolution of 0.9 and 4.6μm, respectively. A clear CARS image of a Nicotiana tabacum L. cv. Bright Yellow 2 cell has been obtained with high vibrational contrast.

© 2008 Optical Society of America

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2005 (3)

2004 (3)

2003 (2)

2002 (1)

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

2001 (1)

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

2000 (1)

1999 (1)

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

1990 (1)

W. Denk, J. H. Strichler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

1987 (1)

J. G. White, W. B. Amos, and M. Fordham, J. Cell Biol. 105, 41 (1987).
[CrossRef] [PubMed]

Alfimov, M. V.

Amos, W. B.

J. G. White, W. B. Amos, and M. Fordham, J. Cell Biol. 105, 41 (1987).
[CrossRef] [PubMed]

Araki, T.

Auguste, J.-L.

Biancalana, F.

Birks, T. A.

Blondy, J.-M.

Book, L. D.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

Campagnola, P. J.

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

Champert, P.-A.

Cheng, J.-X.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

Cicerone, M. T.

Cicerone, T. M.

Conderc, V.

Couderc, V.

Courdec, V.

Denk, W.

W. Denk, J. H. Strichler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Dudley, J. M.

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

Eggleton, B. J.

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[CrossRef]

Fedotov, A. B.

Foehly, C.

Fordham, M.

J. G. White, W. B. Amos, and M. Fordham, J. Cell Biol. 105, 41 (1987).
[CrossRef] [PubMed]

Frevrier, S.

Grossard, L.

Grossard, N.

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

Hamaguchi, H.

Hashimoto, M.

Hilligsoe, K. M.

Huss, G.

Ivanov, A. A.

Joly, N.

Kano, H.

Kawata, S.

Kee, T. W.

Keiding, S. R.

Knight, J. C.

Labonte, L.

Larsen, J. J.

Lee, Y. J.

Leproux, P.

Lesvigne, C.

Lewis, A.

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

Liu, Y.

Loew, L. M.

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

Maillotte, H.

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

Mitrokhin, V. P.

Nerin, P.

Okuno, M.

Paulsen, H. N.

Petrov, G. I.

Pioger, P.-H.

Provino, L.

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

Roy, P.

Russell, P.

P. Russell, Science 299, 358 (2003).
[CrossRef] [PubMed]

Russell, P. St. J.

Strichler, J. H.

W. Denk, J. H. Strichler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Thogersen, J.

Tombelaine, V.

Volkmer, A.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

Wadswprth, W. J.

Webb, W. W.

W. Denk, J. H. Strichler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

Wei, M.

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

White, J. G.

J. G. White, W. B. Amos, and M. Fordham, J. Cell Biol. 105, 41 (1987).
[CrossRef] [PubMed]

Windeler, R. A.

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

Xie, X. S.

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

Yakovlev, V. V.

Zheltikov, A. M.

Anal. Chem. (1)

H. Kano and H. Hamaguchi, Anal. Chem. 79, 8967 (2007).
[CrossRef] [PubMed]

Biophys. J. (1)

P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3331 (1999).
[CrossRef]

Electron. Lett. (1)

L. Provino, J. M. Dudley, H. Maillotte, N. Grossard, R. A. Windeler, and B. J. Eggleton, Electron. Lett. 37, 558 (2001).
[CrossRef]

J. Cell Biol. (1)

J. G. White, W. B. Amos, and M. Fordham, J. Cell Biol. 105, 41 (1987).
[CrossRef] [PubMed]

J. Phys. Chem. B (1)

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 106, 8493 (2002).
[CrossRef]

Opt. Express (5)

Opt. Lett. (6)

Science (2)

P. Russell, Science 299, 358 (2003).
[CrossRef] [PubMed]

W. Denk, J. H. Strichler, and W. W. Webb, Science 248, 73 (1990).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) Experimental setup of the subnanosecond multiplex CARS microspectroscopy: WP, half-wave-plate; BS, beam splitter; LF, long-wavelength-pass filter; EF, 1064 nm edge filter; SP, short-wavelength-pass filter. (b) Typical spectral profile of the subnanosecond NIR supercontinuum for ω 2 of the CARS process. The inset is the photograph of the photonic crystal fiber changing its color gradually from blue to orange by the propagation of the supercontinuum.

Fig. 2
Fig. 2

Intensity-corrected multiplex CARS spectra of a polystyrene bead in (a) 500 4000 cm 1 and in (b) fingerprint region. CARS images of a polystyrene bead with diameter of 3 μ m at the Raman shifts of (c) 992, (d) 1572, and (e) 1900 cm 1 .

Fig. 3
Fig. 3

(a) Intensity-corrected multiplex CARS spectrum of BY2 cells. (b) CARS image of BY2 cells at a Raman shift at approximately 2900 cm 1 .

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