A simple time-domain optical method for estimating the depth and lifetime of fluorescent inclusions in a turbid medium is described. We demonstrate the method for depth and lifetime estimation of a fluorescent inclusion directly by fitting a monoexponential decay of the temporal position of the temporal point-spread function and the measurement of its maximum temporal position . Since both of these measurements are intensity independent, this method provides a robust and efficient approach. This method is validated with experimental data.
© 2008 Optical Society of AmericaFull Article | PDF Article