Abstract

We present a method for multispectral imaging. This method uses color CCD cameras with a multiple-bandpass filter, which modifies the spectral response of the cameras used and enables concurrent acquisition of multiple images at defined spectral bands. We experimentally demonstrate methodological feasibility using two color CCD cameras and a polychroic mirror to simultaneously capture eight spectral bands. We discuss how the method developed is well suited for multispectral applications of transient phenomena or for real-time measurements.

© 2008 Optical Society of America

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  1. R. M. Levenson and J. R. Mansfield, Cytometry 69, 748 (2006).
    [CrossRef] [PubMed]
  2. Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
    [CrossRef] [PubMed]
  3. Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.
  4. R. Levenson and D. Farkas, Proc. SPIE 2983, 123 (1997).
    [CrossRef]
  5. C. D. Tran and R. J. Furlan, Anal. Chem. 64, 2775 (1992).
    [CrossRef] [PubMed]
  6. K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
    [CrossRef] [PubMed]
  7. R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
    [CrossRef] [PubMed]
  8. S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).
  9. J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
    [CrossRef]
  10. R. Shogenji, Y. Kitamura, K. Yamada, S. Miyatake, and J. Tanida, Opt. Express 12, 1643 (2004).
    [CrossRef] [PubMed]

2006 (1)

R. M. Levenson and J. R. Mansfield, Cytometry 69, 748 (2006).
[CrossRef] [PubMed]

2004 (1)

2002 (1)

Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
[CrossRef] [PubMed]

2001 (1)

J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
[CrossRef]

1999 (2)

R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
[CrossRef] [PubMed]

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

1997 (1)

R. Levenson and D. Farkas, Proc. SPIE 2983, 123 (1997).
[CrossRef]

1992 (1)

C. D. Tran and R. J. Furlan, Anal. Chem. 64, 2775 (1992).
[CrossRef] [PubMed]

1987 (1)

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Balchum, O. J.

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Brodbeck, K. J.

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Buckwald, R.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Cabib, D.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Edwards, D. A.

R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
[CrossRef] [PubMed]

Farkas, D.

R. Levenson and D. Farkas, Proc. SPIE 2983, 123 (1997).
[CrossRef]

Frewin, T.

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Furlan, R. J.

C. D. Tran and R. J. Furlan, Anal. Chem. 64, 2775 (1992).
[CrossRef] [PubMed]

Garini, Y.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Haerendel, G.

J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
[CrossRef]

Haraguchi, T.

Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
[CrossRef] [PubMed]

Hiraoka, Y.

Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
[CrossRef] [PubMed]

Katzir, N.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Kitamura, Y.

Lerner, J. M.

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

Levenson, R.

R. Levenson and D. Farkas, Proc. SPIE 2983, 123 (1997).
[CrossRef]

Levenson, R. M.

R. M. Levenson and J. R. Mansfield, Cytometry 69, 748 (2006).
[CrossRef] [PubMed]

Lummerzheim, D.

J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
[CrossRef]

Malik, Z.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Mansfield, J. R.

R. M. Levenson and J. R. Mansfield, Cytometry 69, 748 (2006).
[CrossRef] [PubMed]

Miyatake, S.

Muller, G.

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

Naber, R. D.

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

Ornberg, R. L.

R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
[CrossRef] [PubMed]

Profio, A. E.

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Semeter, J.

J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
[CrossRef]

Shimi, T.

Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
[CrossRef] [PubMed]

Shogenji, R.

Soenksen, D. G.

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

Tanida, J.

Tran, C. D.

C. D. Tran and R. J. Furlan, Anal. Chem. 64, 2775 (1992).
[CrossRef] [PubMed]

Vari, S. G.

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

Woerner, B. M.

R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
[CrossRef] [PubMed]

Yamada, K.

Anal. Chem. (1)

C. D. Tran and R. J. Furlan, Anal. Chem. 64, 2775 (1992).
[CrossRef] [PubMed]

Cell Struct. Funct (1)

Y. Hiraoka, T. Shimi, and T. Haraguchi, Cell Struct. Funct 27, 367 (2002).
[CrossRef] [PubMed]

Cytometry (1)

R. M. Levenson and J. R. Mansfield, Cytometry 69, 748 (2006).
[CrossRef] [PubMed]

J. Atmos. Sol.-Terr. Phys. (1)

J. Semeter, D. Lummerzheim, and G. Haerendel, J. Atmos. Sol.-Terr. Phys. 63, 1981 (2001).
[CrossRef]

J. Histochem. Cytochem. (1)

R. L. Ornberg, B. M. Woerner, and D. A. Edwards, J. Histochem. Cytochem. 47, 1307 (1999).
[CrossRef] [PubMed]

Med. Phys. (1)

K. J. Brodbeck, A. E. Profio, T. Frewin, and O. J. Balchum, Med. Phys. 14, 637 (1987).
[CrossRef] [PubMed]

Opt. Express (1)

Proc. SPIE (1)

R. Levenson and D. Farkas, Proc. SPIE 2983, 123 (1997).
[CrossRef]

Stud. Health Technol. Inform. (1)

S. G. Vari, G. Muller, J. M. Lerner, and R. D. Naber, Stud. Health Technol. Inform. 68, 211 (1999).

Other (1)

Y. Garini, N. Katzir, D. Cabib, R. Buckwald, D. G. Soenksen, and Z. Malik, in Fluorescence Imaging Spectroscopy and Microscopy, X.F.Wang and B.Herman, eds. (John Wiley and Sons, 1996), Vol. 137, pp. 84-124.

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Figures (3)

Fig. 1
Fig. 1

(a) Every group of four adjacent pixels of the CCD is covered by a set of CMYG microfilters. (b) QBPF transmits light in four narrow spectral bands. (c) Sensitivity of the CCD pixels with (thick solid curve) and without QBPF (thin dashed curve).

Fig. 2
Fig. 2

(a) Schematic of the experimental setup. (b) Transmittance of the low-pass filter (thin solid curve) and transmittance (thick solid curve) and reflectance (dashed curve) of polychroic mirror when combined with the low-pass filter.

Fig. 3
Fig. 3

(a) Measurements of reflectance of the checkerboard samples (open diamonds) and reflectance spectra (solid curve). Each graph corresponds to a different color sample: (b) color image of leaves on a plastic background with similar color, (c) map of the leaf reflectance spectrum, and (d) map of the reflectance spectrum of the background material.

Equations (6)

Equations on this page are rendered with MathJax. Learn more.

S X = w X 1 ( I λ 1 ) + w X 2 ( I λ 2 ) + w X 3 ( I λ 3 ) + w X 4 ( I λ 4 ) .
[ S C S M S Y S G ] = [ w C 1 w C 2 w C 3 w C 4 w M 1 w M 2 w M 3 w M 4 w Y 1 w Y 2 w Y 3 w Y 4 w G 1 w G 2 w G 3 w G 4 ] [ I λ 1 I λ 2 I λ 3 I λ 4 ] ,
S = W I ,
I = W 1 ( S ) .
S X , j = i = 1 l w X , i I λ i , j ,
W = S ( I 1 ) .

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