Abstract

A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10μm wide. The elastic-scattering signal was analyzed by generalized Lorenz–Mie theory, representing what we believe to be the first experimental validation of the theory’s prediction of angular backscatter from single spheres. The microscope system exhibits 3nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned.

© 2008 Optical Society of America

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