Abstract

We demonstrate label-free molecule detection by using an integrated biosensor based on a Si3N4SiO2 slot-waveguide microring resonator. Bovine serum albumin (BSA) and anti-BSA molecular binding events on the sensor surface are monitored through the measurement of resonant wavelength shifts with varying biomolecule concentrations. The biosensor exhibited sensitivities of 1.8 and 3.2nm(ngmm2) for the detection of anti-BSA and BSA, respectively. The estimated detection limits are 28 and 16pgmm2 for anti-BSA and BSA, respectively, limited by wavelength resolution.

© 2008 Optical Society of America

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A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

C. A. Barrios, K. B. Gylfason, B. Sanchez, A. Griol, H. Sohlström, M. Holgado, and R. Casquel, Opt. Lett. 32, 3080 (2007).
[CrossRef] [PubMed]

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A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

C.-Y. Chao, W. Fung, and L. J. Guo, IEEE J. Sel. Top. Quantum Electron. 12, 134 (2006).
[CrossRef]

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H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

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[CrossRef]

2004 (2)

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[CrossRef] [PubMed]

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

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J. P. Ou, S. T. H. Chan, and W. S. B. Yeung, J. Chromatogr., B: Biomed. Appl. 731, 389 (1999).
[CrossRef]

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1996 (1)

A Desai, T.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Aldridge, J. C.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Almeida, V.

Anthes-Washburn, M.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Armani, A. M.

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Barrios, C. A.

Boyd, R. W.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Casquel, R.

Chan, S. T. H.

J. P. Ou, S. T. H. Chan, and W. S. B. Yeung, J. Chromatogr., B: Biomed. Appl. 731, 389 (1999).
[CrossRef]

Chao, C.-Y.

C.-Y. Chao, W. Fung, and L. J. Guo, IEEE J. Sel. Top. Quantum Electron. 12, 134 (2006).
[CrossRef]

Chase, C.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Chbouki, N.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Chu, S.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Fabricius, N.

Fajardo, J. E.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Flagan, R. C.

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Fraser, S. E.

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Fung, W.

C.-Y. Chao, W. Fung, and L. J. Guo, IEEE J. Sel. Top. Quantum Electron. 12, 134 (2006).
[CrossRef]

Garstecki, P.

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

Gill, D.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Goldberg, B. B.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Griol, A.

Guo, L. J.

C.-Y. Chao, W. Fung, and L. J. Guo, IEEE J. Sel. Top. Quantum Electron. 12, 134 (2006).
[CrossRef]

Gylfason, K. B.

Han, M.-K.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Harris, R. D.

Hocdé, S.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Holgado, M.

Hollenbach, U.

Hong, M.-Y.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Hryniewicz, J.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Ingenhoff, J.

Kim, H.-S.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Kim, Y.-J.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

King, O.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Kriebel, J. K.

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

Ksendzov, A.

Kulkarni, R. P.

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Lee, D.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Leeb, J.-H.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Lepeshkin, N. N.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Lin, Y.

Lipson, M.

Little, B. E.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Luff, B. J.

Mayer, M.

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

Ou, J. P.

J. P. Ou, S. T. H. Chan, and W. S. B. Yeung, J. Chromatogr., B: Biomed. Appl. 731, 389 (1999).
[CrossRef]

Piehler, J.

Popat, K. C.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Sanchez, B.

Schweinsberg, A.

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Seok, H.-J.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Sohlström, H.

Ünlü, M. S.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Vahala, K. J.

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Van, V.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Whitesides, G. M.

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

Wilkinson, J. S.

Xu, Q.

Yalçin, A.

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

Yang, J.

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

Yeung, W. S. B.

J. P. Ou, S. T. H. Chan, and W. S. B. Yeung, J. Chromatogr., B: Biomed. Appl. 731, 389 (1999).
[CrossRef]

Yoob, J.-S.

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Anal. Biochem. (1)

H.-J. Seok, M.-Y. Hong, Y.-J. Kim, M.-K. Han, D. Lee, J.-H. Leeb, J.-S. Yoob, and H.-S. Kim, Anal. Biochem. 337, 294 (2005).
[CrossRef] [PubMed]

Angew. Chem., Int. Ed. (1)

J. Yang, M. Mayer, J. K. Kriebel, P. Garstecki, and G. M. Whitesides, Angew. Chem., Int. Ed. 43, 1555 (2004).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron. (2)

A. Yalçin, K. C. Popat, J. C. Aldridge, T. A Desai, J. Hryniewicz, N. Chbouki, B. E. Little, O. King, V. Van, S. Chu, D. Gill, M. Anthes-Washburn, M. S. Ünlü, and B. B. Goldberg, IEEE J. Sel. Top. Quantum Electron. 12, 148 (2006).
[CrossRef]

C.-Y. Chao, W. Fung, and L. J. Guo, IEEE J. Sel. Top. Quantum Electron. 12, 134 (2006).
[CrossRef]

J. Chromatogr., B: Biomed. Appl. (1)

J. P. Ou, S. T. H. Chan, and W. S. B. Yeung, J. Chromatogr., B: Biomed. Appl. 731, 389 (1999).
[CrossRef]

J. Lightwave Technol. (1)

Opt. Lett. (4)

Science (1)

A. M. Armani, R. P. Kulkarni, S. E. Fraser, R. C. Flagan, and K. J. Vahala, Science 317, 783 (2007).
[CrossRef] [PubMed]

Sens. Actuators B (1)

A. Schweinsberg, S. Hocdé, N. N. Lepeshkin, R. W. Boyd, C. Chase, and J. E. Fajardo, Sens. Actuators B 123, 727 (2007).
[CrossRef]

Other (1)

Swiss-Prot database, Swiss Institute for Bioinformatics (http://us.expasy.org/sprot/sprot-top.html).

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic cross section of the Si 3 N 4 SiO 2 ring slot-waveguide used for optical sensing of biomolecules. Triangles and Y-shaped symbols represent antigen and antibody molecules, respectively. (b) Calculated quasi-TE mode of the ring slot-waveguide turning to the left ( x axis) with a radius of curvature of 70 μ m and an operation wavelength of 1.3 μ m .

Fig. 2
Fig. 2

Normalized transmission spectra of the slot-waveguide microring resonator for TE-polarization for different anti-BSA concentrations: S1 ( 0.4 μ g ml ) , S2 ( 0.44 μ g ml ) , S3 ( 2.04 μ g ml ) , and S4 ( 3.64 μ g ml ) .

Fig. 3
Fig. 3

Resonance wavelength shift versus anti-BSA concentration. δ λ antiBSA = 3 nm is the spectral shift at anti-BSA surface saturation.

Fig. 4
Fig. 4

Resonance wavelength shift versus BSA concentration. δ λ BSA = 2.45 nm is the spectral shift at BSA surface saturation.

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