Abstract

Photothermal response (PTR) is an established pump and probe technique for real-time sensing of biological assays. Continuous and selective PTR monitoring is difficult owing to the Brownian motion changing the relative position of the target with respect to the beams. Integration of laser trapping with PTR is proposed as a solution. The proposed method is verified on red polystyrene microparticles. PTR is continuously monitored for 30min. Results show that the mean relaxation time variation of the acquired signals is less than 5%. The proposed method is then applied to human red blood cells for continuous and selective PTR.

© 2008 Optical Society of America

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References

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  1. Q. He, R. Vyas, and R. Gupta, Appl. Opt. 36, 7046 (1997).
    [CrossRef]
  2. H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
    [CrossRef]
  3. D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.
  4. S. Vasudevan and G. Chen, The OSA Topical Conference on Nanophotonics (Optical Society of America, 2007).
  5. D. Lapotko, Cytometry, Part A 58A, 111 (2004).
    [CrossRef]
  6. V. P. Zharov, IEEE J. Sel. Top. Quantum Electron. 11, 733 (2005).
    [CrossRef]
  7. K. J. Aufderheide, Micron 39, 71 (2008).
    [CrossRef]
  8. D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
    [CrossRef]
  9. D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
    [CrossRef]
  10. D. Lasne, G. A. Blab, F. De Giorgi, F. Ichas, B. Lounis, and L. Cognet, Opt. Express 15, 14184 (2007).
    [CrossRef] [PubMed]
  11. K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
    [CrossRef]
  12. A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
    [CrossRef]

2008 (1)

K. J. Aufderheide, Micron 39, 71 (2008).
[CrossRef]

2007 (2)

S. Vasudevan and G. Chen, The OSA Topical Conference on Nanophotonics (Optical Society of America, 2007).

D. Lasne, G. A. Blab, F. De Giorgi, F. Ichas, B. Lounis, and L. Cognet, Opt. Express 15, 14184 (2007).
[CrossRef] [PubMed]

2005 (2)

V. P. Zharov, IEEE J. Sel. Top. Quantum Electron. 11, 733 (2005).
[CrossRef]

D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
[CrossRef]

2004 (2)

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

D. Lapotko, Cytometry, Part A 58A, 111 (2004).
[CrossRef]

2002 (1)

D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.

2000 (1)

D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
[CrossRef]

1997 (1)

1995 (1)

H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
[CrossRef]

1970 (1)

A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Allaway, D.

D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
[CrossRef]

Ashkin, A.

A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Aufderheide, K. J.

K. J. Aufderheide, Micron 39, 71 (2008).
[CrossRef]

Blab, G. A.

Block, S. M.

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

Chen, G.

S. Vasudevan and G. Chen, The OSA Topical Conference on Nanophotonics (Optical Society of America, 2007).

Cognet, L.

De Giorgi, F.

Gordiyko, E.

D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.

Grigoropoulos, C. P.

H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
[CrossRef]

Gupta, R.

He, Q.

Ichas, F.

Lapotko, D.

D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
[CrossRef]

D. Lapotko, Cytometry, Part A 58A, 111 (2004).
[CrossRef]

D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.

Lasne, D.

Lounis, B.

Lukianova, E.

D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
[CrossRef]

Neuman, K. C.

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

Park, H. K.

H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
[CrossRef]

Poole, P. S.

D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
[CrossRef]

Romanovskaya, T. Y.

D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.

Schofield, N. A.

D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
[CrossRef]

Shnip, A.

D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
[CrossRef]

Tam, A. C.

H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
[CrossRef]

Vasudevan, S.

S. Vasudevan and G. Chen, The OSA Topical Conference on Nanophotonics (Optical Society of America, 2007).

Vyas, R.

Zharov, V. P.

V. P. Zharov, IEEE J. Sel. Top. Quantum Electron. 11, 733 (2005).
[CrossRef]

Appl. Opt. (1)

Biotechnol. Lett. (1)

D. Allaway, N. A. Schofield, and P. S. Poole, Biotechnol. Lett. 22, 887 (2000).
[CrossRef]

Cytometry, Part A (1)

D. Lapotko, Cytometry, Part A 58A, 111 (2004).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron. (1)

V. P. Zharov, IEEE J. Sel. Top. Quantum Electron. 11, 733 (2005).
[CrossRef]

Int. J. Thermophys. (1)

H. K. Park, C. P. Grigoropoulos, and A. C. Tam, Int. J. Thermophys. 16, 973 (1995).
[CrossRef]

J. Biomed. Opt. (1)

D. Lapotko, A. Shnip, and E. Lukianova, J. Biomed. Opt. 10, 014006 (2005).
[CrossRef]

Micron (1)

K. J. Aufderheide, Micron 39, 71 (2008).
[CrossRef]

Opt. Express (1)

Phys. Rev. Lett. (1)

A. Ashkin, Phys. Rev. Lett. 24, 156 (1970).
[CrossRef]

Rev. Sci. Instrum. (1)

K. C. Neuman and S. M. Block, Rev. Sci. Instrum. 75, 2787 (2004).
[CrossRef]

Other (2)

D. Lapotko, T. Y. Romanovskaya, and E. Gordiyko, Functional Monitoring and Drug-Tissue Interaction (Proc. SPIE, 2002), p. 314.

S. Vasudevan and G. Chen, The OSA Topical Conference on Nanophotonics (Optical Society of America, 2007).

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Figures (6)

Fig. 1
Fig. 1

Continuous PTR of a single red polystyrene particle acquired at different time intervals (X axis, 5 μ s div ; Y axis, (a) 20 mV div , (b) and (c) 5 mV div ).

Fig. 2
Fig. 2

PTR simulation by changing target position (probe beam diameter, 15 μ m ; target size, 1 μ m ). (a) Target is at the center of the beams. (b) Target is offset from the center by 3 μ m .

Fig. 3
Fig. 3

Experimental setup of integrated PTR and laser trapping.

Fig. 4
Fig. 4

Results of a trapped red polystyrene particle (Y axis, 10 mV div , X axis, 5 μ s div ) at different time intervals.

Fig. 5
Fig. 5

PTR’s relaxation time of four sets of a single red polystyrene particle at five different time intervals (pump energy, 0.5 μ J ; probe power, 4.3 mW ).

Fig. 6
Fig. 6

Continuous PTR of an isolated RBC at different time intervals (pump energy, 0.25 μ J ; probe power, 1.5 mW ). (a) Image of a group of RBCs, (b) isolated RBC from the group, and (c) PTR of the isolated RBC at different time intervals.

Tables (1)

Tables Icon

Table 1 PTR for Varying Probe Beam Powers for a Group of Six Red Polystyrene Particles

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