Abstract

We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

© 2008 Optical Society of America

Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei

Cyrus Amoozegar, Michael G. Giacomelli, Justin D. Keener, Kevin J. Chalut, and Adam Wax
Appl. Opt. 48(10) D20-D25 (2009)

Application of Mie theory to determine the structure of spheroidal scatterers in biological materials

Justin D. Keener, Kevin J. Chalut, John W. Pyhtila, and Adam Wax
Opt. Lett. 32(10) 1326-1328 (2007)

Application of Mie theory to assess structure of spheroidal scattering in backscattering geometries

Kevin J. Chalut, Michael G. Giacomelli, and Adam Wax
J. Opt. Soc. Am. A 25(8) 1866-1874 (2008)

Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering

Michael Giacomelli, Yizheng Zhu, John Lee, and Adam Wax
Opt. Express 18(14) 14616-14626 (2010)

Field-based angle-resolved light-scattering study of single live cells

Wonshik Choi, Chung-Chieh Yu, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld
Opt. Lett. 33(14) 1596-1598 (2008)