Abstract

A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial–spectral information within an object. We demonstrate this imaging system’s ability to obtain multiple depth-resolved fluorescence images simultaneously.

© 2008 Optical Society of America

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