Abstract

Calibration of the diffuse reflectance spectrum for instrument response and time-dependent fluctuation as well as interdevice variations is complicated, time consuming, and potentially inaccurate. We describe a novel fiber optic probe with a real-time self-calibration capability that can be used for tissue optical spectroscopy. The probe was tested in a number of liquid phantoms over a relevant range of tissue optical properties. Absorption and scattering coefficients are extracted with an average absolute error and standard deviation of 6.9%±7.2% and 3.5%±1.5%, respectively.

© 2008 Optical Society of America

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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2006 (1)

2004 (1)

I. J. Bigio and S. G. Bown, Cancer Biol. Ther. 3, 259 (2004).
[CrossRef] [PubMed]

2003 (2)

U. Utzinger and R. R. Richards-Kortum, J. Biomed. Opt. 8, 121 (2003).
[CrossRef] [PubMed]

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

2002 (1)

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

2001 (1)

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

1999 (1)

Atkinson, E. N.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

Backman, V.

Bevilacqua, F.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Bigio, I. J.

I. J. Bigio and S. G. Bown, Cancer Biol. Ther. 3, 259 (2004).
[CrossRef] [PubMed]

Blast, R. C.

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Bown, S. G.

I. J. Bigio and S. G. Bown, Cancer Biol. Ther. 3, 259 (2004).
[CrossRef] [PubMed]

Brewer, M.

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Chang, S. K.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

Charvet, I.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Dam, J. V.

Depeursinge, C.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Feld, M. S.

Fitzmaurice, M.

Follen, M.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Gershenson, D.

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Malpica, A.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

Manoharan, R.

Marquet, P.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Meda, P.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Mirabal, Y. N.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

Ory, G.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Palmer, G. M.

Perelman, L. T.

Ramanujam, N.

Richards-Kortum, R.

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Richards-Kortum, R. R.

U. Utzinger and R. R. Richards-Kortum, J. Biomed. Opt. 8, 121 (2003).
[CrossRef] [PubMed]

Silva, E.

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

St Ghislain, M.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Thueler, P.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Utzinger, U.

U. Utzinger and R. R. Richards-Kortum, J. Biomed. Opt. 8, 121 (2003).
[CrossRef] [PubMed]

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

Vermeulen, B.

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Zonios, G.

Appl. Opt. (2)

Cancer Biol. Ther. (1)

I. J. Bigio and S. G. Bown, Cancer Biol. Ther. 3, 259 (2004).
[CrossRef] [PubMed]

J. Biomed. Opt. (3)

U. Utzinger and R. R. Richards-Kortum, J. Biomed. Opt. 8, 121 (2003).
[CrossRef] [PubMed]

P. Thueler, I. Charvet, F. Bevilacqua, M. St Ghislain, G. Ory, P. Marquet, P. Meda, B. Vermeulen, and C. Depeursinge, J. Biomed. Opt. 8, 495 (2003).
[CrossRef] [PubMed]

Y. N. Mirabal, S. K. Chang, E. N. Atkinson, A. Malpica, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 7, 587 (2002).
[CrossRef] [PubMed]

Lasers Surg. Med. (1)

U. Utzinger, M. Brewer, E. Silva, D. Gershenson, R. C. Blast, Jr., M. Follen, and R. Richards-Kortum, Lasers Surg. Med. 28, 56 (2001).
[CrossRef] [PubMed]

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Figures (4)

Fig. 1
Fig. 1

Block diagram of the DRS system and self-calibrating fiber optic probe (A, illumination adaptor; B, distal end of the probe; C, collection adaptor; and D, termination of the calibration fibers).

Fig. 2
Fig. 2

Correction for illumination intensity fluctuations: (a) raw spectra from the mirror for different levels of attenuation by an ND filter and no attenuation, (b) ratio of the mirror and self-calibration spectra under different attenuation conditions.

Fig. 3
Fig. 3

Raw spectra of phantom 1, 7, and 17 divided by the Spectralon puck spectrum (solid curve) and by the self-calibration spectrum (circles).

Fig. 4
Fig. 4

Extracted versus expected phantom optical properties: (a) absorption coefficient, (b) reduced scattering coefficient.

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