Abstract

We present a numerical technique for extended focused imaging and three-dimensional analysis of a microparticle field observed in a digital holographic microscope working in transmission. The three-dimensional localization of objects is performed using the local focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, to obtain the depth map and a synthetic image in which all objects are refocused independent from their refocusing distance. A successful application of this technique in the analysis of the microgravity particle flow experiment is presented.

© 2008 Optical Society of America

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2008 (1)

2007 (3)

2006 (4)

2005 (1)

2004 (2)

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

M. Liebling and M. Unser, J. Opt. Soc. Am. A 21, 2424 (2004).
[CrossRef]

2003 (1)

2001 (1)

2000 (1)

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

1999 (1)

1989 (1)

J. Gillespie and R. King, Pattern Recogn. Lett. 9, 19 (1989).
[CrossRef]

1986 (1)

Alfieri, D.

Badizadegan, K.

Benkouider, A.

Bevilacqua, F.

Buraga-Lefebvre, C.

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Cai, L.

Callens, N.

F. Dubois, N. Callens, C. Yourassowsky, M. Hoyos, P. Kurowski, and O. Monnom, Appl. Opt. 45, 864 (2006).
[CrossRef] [PubMed]

F. Dubois, C. Schockaert, N. Callens, and C. Yourassowsky, Opt. Express 14, 5895 (2006).
[CrossRef] [PubMed]

N. Callens, Ph.D. dissertation, “Développment, étude expérimentale et visualisation par holographie digitale de mini-séparateurs fluidiques (STEP-SPLITT) en vue de la séparation d'objects de taille micrométrique” (Université Paris VI and Université Libre de Bruxelles, 2005).

Castro, A.

Coëtmellec, S.

D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003).
[CrossRef] [PubMed]

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Coppola, G.

Cuche, E.

Daneshpanah, M.

Dasari, R.

Davis, C.

De Nicola, S.

Depeursinge, C.

Dubois, F.

Feld, M.

Ferraro, P.

Finizio, A.

Frauel, Y.

Gillespie, J.

J. Gillespie and R. King, Pattern Recogn. Lett. 9, 19 (1989).
[CrossRef]

Grilli, S.

Hennelly, B.

Hoyos, M.

Hu, Q.

Javidi, B.

Jin, H.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

Katz, J.

King, R.

J. Gillespie and R. King, Pattern Recogn. Lett. 9, 19 (1989).
[CrossRef]

Kreis, T.

Kurowski, P.

Lebrun, D.

D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003).
[CrossRef] [PubMed]

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Li, W.

Li, Y.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

Liebling, M.

Loomis, N.

Ma, L.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

Malek, M.

Malkiel, E.

McDonald, J.

McElhinney, C.

Monnom, O.

Moon, I.

Naughton, T.

Özkul, C.

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Park, Y.

Pierattini, G.

Popescu, G.

Schockaert, C.

F. Dubois, C. Schockaert, N. Callens, and C. Yourassowsky, Opt. Express 14, 5895 (2006).
[CrossRef] [PubMed]

C. Schockaert, Ph.D. dissertation “Three-dimensional object analysis and tracking by digital holography microscopy” (Université Libre de Bruxelles, 2006).

Sheng, J.

Striano, V.

Unser, M.

Wang, H.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

Yeom, S.

Yourassowsky, C.

Yu, L.

Appl. Opt. (3)

J. Opt. A Pure Appl. Opt. (1)

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004).
[CrossRef]

J. Opt. Soc. Am. A (4)

Opt. Express (4)

Opt. Lasers Eng. (1)

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Opt. Lett. (3)

Pattern Recogn. Lett. (1)

J. Gillespie and R. King, Pattern Recogn. Lett. 9, 19 (1989).
[CrossRef]

Other (2)

C. Schockaert, Ph.D. dissertation “Three-dimensional object analysis and tracking by digital holography microscopy” (Université Libre de Bruxelles, 2006).

N. Callens, Ph.D. dissertation, “Développment, étude expérimentale et visualisation par holographie digitale de mini-séparateurs fluidiques (STEP-SPLITT) en vue de la séparation d'objects de taille micrométrique” (Université Paris VI and Université Libre de Bruxelles, 2005).

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Figures (3)

Fig. 1
Fig. 1

Demonstration of the local refocusing algorithm: (a) original out-of-focus intensity retrieved from the digital hologram, (b) synthetic EFI. In that example particles have a diameter of 5 μ m , pixel size is 0.33 μ m , and the ROI is 31 × 31   pixels large.

Fig. 2
Fig. 2

Comparison of the digitally refocused DHM images of 7 μ m latex particles with a synthetic EFI created using various sizes of ROIs. (a) Original intensity image, (b) right particle in focus ( d = 18 μ m ) , (c) left particle in focus ( d = 115 μ m ) , EFI images with a ROI of (d) 25 × 25 , (e) 51 × 51 , and (f) 75 × 75   pixels . Pixel size is 0.33 μ m .

Fig. 3
Fig. 3

Particle detection in the hologram recorded in a microparticle resuspension experiment under microgravity conditions [19]: (a) original intensity reconstructed from the hologram (field of view: 330 μ m × 330 μ m ), (b) synthetic EFI, (c) result of image segmentation and detection of particles, (d) refocusing distance versus diameter of the detected particles.

Equations (2)

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M d = u d ( x , y ) d x d y .
M d L = ROI u d ( x , y ) d x d y ROI u d ( x , y ) 2 d x d y .

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