Abstract
We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75° to 90°. We demonstrate in vivo detection of blood flow in vessels as small as in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent.
© 2008 Optical Society of America
Full Article | PDF ArticleOSA Recommended Articles
Kenneth K. C. Lee, Adrian Mariampillai, Joe X. Z. Yu, David W. Cadotte, Brian C. Wilson, Beau A. Standish, and Victor X. D. Yang
Biomed. Opt. Express 3(7) 1557-1564 (2012)
Leigh Conroy, Ralph S. DaCosta, and I. Alex Vitkin
Opt. Lett. 37(15) 3180-3182 (2012)
Reza Motaghiannezam and Scott Fraser
Biomed. Opt. Express 3(3) 503-521 (2012)