Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy

Abstract

A factor that limits the use of multiphoton microscopy (MPM) in clinical and preclinical studies is the lack of a compact and flexible probe. We report on a miniaturized MPM probe employing a microelectromechanical system (MEMS) scanning mirror and a double-clad photonic crystal fiber (DCPCF). The use of a MEMS mirror and a DCPCF provides many advantages, such as size reduction, rapid and precise scanning, efficient delivery of short pulses, and high collection efficiency of fluorescent signals. The completed probe was 1 cm in outer diameter and $14\mathrm{cm}$ in length. The developed probe was integrated into an MPM system and used to image fluorescent beads, paper, and biological specimens.

© 2008 Optical Society of America

Miniaturized all-optical photoacoustic microscopy based on microelectromechanical systems mirror scanning

Sung-Liang Chen, Zhixing Xie, Tao Ling, L. Jay Guo, Xunbin Wei, and Xueding Wang
Opt. Lett. 37(20) 4263-4265 (2012)

Microelectromechanical systems scanning-mirror-based handheld probe for fluorescence molecular tomography

Bin He, Lei Xi, Sean R. Samuelson, Huikai Xie, Lily Yang, and Huabei Jiang
Appl. Opt. 51(20) 4678-4683 (2012)

Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser

Lin Huang, Arthur K. Mills, Yuan Zhao, David J. Jones, and Shuo Tang
Biomed. Opt. Express 7(5) 1948-1956 (2016)

In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

Wibool Piyawattanametha, Eric D. Cocker, Laurie D. Burns, Robert P. J. Barretto, Juergen C. Jung, Hyejun Ra, Olav Solgaard, and Mark J. Schnitzer
Opt. Lett. 34(15) 2309-2311 (2009)

Axial beam scanning in multiphoton microscopy with MEMS-based actuator

Xiyu Duan, Haijun Li, Xue Li, Kenn R. Oldham, and Thomas D. Wang
Opt. Express 25(3) 2195-2205 (2017)