Abstract

We use a parallel frequency-domain optical coherence tomography (FDOCT) system to generate a scatter-mode image of the hamster cheek pouch epithelium. To our knowledge, this is the first optical coherence tomography (OCT) image of a biological sample obtained using a thermal light source in the frequency domain. The system employs an imaging spectrometer to acquire depth-resolved profiles from adjacent spatial points without the need for any scanning. To enable this imaging modality, we have considered that signals originating from multiple depths combine in a different manner in FDOCT compared to time-domain optical coherence tomography (TDOCT). Because a multicomponent FDOCT signal is a coherent sum, it is necessary to limit the number of modes that contribute to the detected signal. Conversely, multicomponent TDOCT signals can be represented as incoherent sums, where increasing the number of modes improves the signal.

© 2008 Optical Society of America

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
    [CrossRef] [PubMed]
  2. A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
    [CrossRef]
  3. B. Grajciar, M. Pircher, A. F. Fercher, and R. A. Leitgeb, Opt. Express 13, 1131 (2005).
    [CrossRef] [PubMed]
  4. L. Vabre, A. Dubois, and A. C. Boccara, Opt. Lett. 27, 530 (2002).
    [CrossRef]
  5. A. Wax, C. H. Yang, and J. A. Izatt, Opt. Lett. 28, 1230 (2003).
    [CrossRef] [PubMed]
  6. R. N. Graf and A. Wax, Opt. Express 13, 4693 (2005).
    [CrossRef]
  7. A. Wax, C. H. Yang, R. R. Dasari, and M. S. Feld, Opt. Lett. 26, 322 (2001).
    [CrossRef]
  8. R. N. Graf and A. Wax, J. Opt. Soc. Am. A 24, 2186 (2007).
    [CrossRef]

2007 (1)

2005 (2)

2003 (1)

2002 (1)

2001 (1)

2000 (1)

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Boccara, A. C.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Dasari, R. R.

Drexler, W.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Dubois, A.

Feld, M. S.

Fercher, A. F.

B. Grajciar, M. Pircher, A. F. Fercher, and R. A. Leitgeb, Opt. Express 13, 1131 (2005).
[CrossRef] [PubMed]

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Graf, R. N.

Grajciar, B.

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hitzenberger, C. K.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Izatt, J. A.

Leitgeb, R.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Leitgeb, R. A.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Moreno-Barriuso, E.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Pircher, M.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Sattmann, H.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Sticker, M.

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Vabre, L.

Wax, A.

Yang, C. H.

J. Opt. Soc. Am. A (1)

Opt. Commun. (1)

A. F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, Opt. Commun. 185, 57 (2000).
[CrossRef]

Opt. Express (2)

Opt. Lett. (3)

Science (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

Schematic of the parallel FDOCT system using a xenon arc-lamp source.

Fig. 2
Fig. 2

(a) Experimental spectra obtained from a scattering standard sample. (b) Depth scans generated from raw spectra in (a). Depth information is degraded by summing spectra before Fourier transforming (solid curve) but preserved by summing channels after Fourier transforming (dashed curve).

Fig. 3
Fig. 3

Parallel FDOCT image of a hamster cheek pouch: E, squamous epithelium; M, mucosa; S, submucosa; and F, tissue fold. Scale bars correspond to 50 μ m vertically and 125 μ m horizontally.

Equations (3)

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S FD ( ω ) = d t n cos ( T n ( ω + ω 0 ) ) exp ( a 2 2 ( ω + ω 0 ) 2 ) exp ( 2 a 2 ( t T n 2 ) 2 ) = a π 2 exp ( a 2 2 ( ω + ω 0 ) 2 ) n cos ( T n ( ω + ω 0 ) ) .
S T D ( T n ) = d t d ω n ( cos ( T n ( ω + ω 0 ) )
+ i sin ( T n ( ω + ω 0 ) ) ) exp ( a 2 2 ( ω + ω 0 ) 2 ) exp ( 2 a 2 ( t T n 2 ) 2 ) = π 2 n exp ( T n 2 2 a 2 ) .

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