Abstract

An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO2 quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO2. The instrument was demonstrated by performing depth-resolved microvascular pO2 measurements of rat cortical vessels down to 120μm below the surface. 2P excitation of porphyrin was confirmed, and measured pO2 values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO2 measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO2 measurement techniques.

© 2008 Optical Society of America

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