Abstract

We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging exhibits spatial and temporal phase stability in the subnanometer range. We also demonstrate the visualization of actin filaments in a fixed cell specimen, which is confirmed by simultaneous multiphoton fluorescence imaging.

© 2007 Optical Society of America

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2006 (3)

2005 (3)

2004 (3)

2002 (1)

1999 (1)

1998 (1)

1997 (1)

D. W. Pierce, N. Hom-Booher, and R. D. Vale, Nature 388, 338 (1997).
[CrossRef] [PubMed]

1994 (1)

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

1993 (2)

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

S. Kostianovski, S. G. Lipson, and E. N. Ribak, Appl. Opt. 32, 4744 (1993).
[CrossRef] [PubMed]

Akkin, T.

Amblard, F.

Badizadegan, K.

Barty, A.

Beaurepaire, E.

Block, S. M.

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

Boppart, S. A.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Bouma, B. E.

Cense, B.

Chen, T. C.

Colomb, T.

Cuche, E.

Dasari, R. R.

Dave, D.

de Boer, J. F.

Deflores, L. P.

Depeursinge, C.

Emery, Y.

Feld, M. S.

Fercher, A. F.

Fujimoto, J. G.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Gotzinger, E.

Hee, M. R.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Hitzenberger, C. K.

Hom-Booher, N.

D. W. Pierce, N. Hom-Booher, and R. D. Vale, Nature 388, 338 (1997).
[CrossRef] [PubMed]

Ikeda, T.

Iwai, H.

Izatt, J. A.

M. V. Sarunic, S. Weinberg, and J. A. Izatt, Opt. Lett. 31, 1462 (2006).
[CrossRef] [PubMed]

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Joo, C.

Kostianovski, S.

Lipson, S. G.

Luo, W.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Magistretti, P. J.

Marks, D. L.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Marquet, P.

Mertz, J.

Milner, T. E.

Moreaux, L.

Nassif, N.

Nugent, K. A.

Owen, G. M.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Paganin, D.

Park, B. H.

Park, Y.

Pierce, D. W.

D. W. Pierce, N. Hom-Booher, and R. D. Vale, Nature 388, 338 (1997).
[CrossRef] [PubMed]

Pircher, M.

Popescu, G.

Ralston, T.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Rappaz, B.

Ribak, E. N.

Roberts, A.

Rylander, H. G.

Sarunic, M. V.

Sattmann, H.

Schmidt, C. F.

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

Schnapp, B. J.

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

Sticker, M.

Svoboda, K.

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

Swanson, E. A.

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Tan, W.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Tearney, G. J.

Vale, R. D.

D. W. Pierce, N. Hom-Booher, and R. D. Vale, Nature 388, 338 (1997).
[CrossRef] [PubMed]

Vaughan, J. C.

Vinegoni, C.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Weinberg, S.

Yun, S. H.

Appl. Opt. (1)

Appl. Phys. Lett. (1)

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Nature (2)

D. W. Pierce, N. Hom-Booher, and R. D. Vale, Nature 388, 338 (1997).
[CrossRef] [PubMed]

K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, Nature 365, 721 (1993).
[CrossRef] [PubMed]

Opt. Express (2)

Y. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Express 14, 8263 (2006).
[CrossRef] [PubMed]

J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Express 19, 590 (1994).

Opt. Lett. (10)

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Figures (3)

Fig. 1
Fig. 1

Schematic of SD-OCP/MPM. HW, half-wave plate; C, collimator; SL, scan lens; TL, tube lens; DM, dichroic mirror; F, filter; M, mirror; L, lens.

Fig. 2
Fig. 2

Spatial and temporal phase stability of SD-OCP–MPM. (a) The 2D phase repeatability map demonstrates σ 0.5 nm repeatability in air. (b) Phase fluctuation for a stationary beam; the standard deviation was 53 pm at a SNR of 63.4 dB .

Fig. 3
Fig. 3

Images of fixed and stained muntjac skin fibroblast cells. (a) The TPF image shows the distribution of actin filaments labeled with Alexa Fluor 488 phalloidin. (b) Intensity and (c) quantitative phase contrast images obtained in reflection with SD-OCPM. The color bar to the right of the phase contrast image denotes the phase distribution in radians. (d) Computed phase difference and (e) 3D representation of the phase image. The scale bar represents 10 μ m .

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