Fluorescence tomography of diffuse media can yield optimal three-dimensional imaging when multiple projections over geometries are captured, compared with limited projection angle systems such as implementations in the slab geometry. We demonstrate how it is possible to perform noncontact, projection fluorescence tomography of mice using CCD-camera-based detection in free space, i.e., in the absence of matching fluids. This approach achieves high spatial sampling of photons propagating through tissue and yields a superior information content data set compared with fiber-based implementations. Reconstruction feasibility using 36 projections in 10° steps is demonstrated in mice.
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