Abstract

A miniature objective lens with a tip diameter of 1.3mm was used for extending the penetration depth of coherent anti-Stokes Raman scattering (CARS) microscopy. Its axial and lateral focal widths were determined to be 11.4 and 0.86μm, respectively, by two-photon excitation fluorescence imaging of 200nm beads at a 735nm excitation wavelength. By inserting the lens tip into a soft gel sample, CARS images of 2μm polystyrene beads 5mm deep from the surface were acquired. The miniature objective was applied to CARS imaging of rat spinal cord white matter with a minimal requirement for surgery.

© 2007 Optical Society of America

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