A miniature objective lens with a tip diameter of was used for extending the penetration depth of coherent anti-Stokes Raman scattering (CARS) microscopy. Its axial and lateral focal widths were determined to be 11.4 and , respectively, by two-photon excitation fluorescence imaging of beads at a excitation wavelength. By inserting the lens tip into a soft gel sample, CARS images of polystyrene beads deep from the surface were acquired. The miniature objective was applied to CARS imaging of rat spinal cord white matter with a minimal requirement for surgery.
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