Abstract

A confocal reflectance microscope has been developed that incorporates a dual-wedge scanner to reduce the size of the device relative to current raster scanning instruments. The scanner is implemented with two prisms that are rotated about the optical axis. Spiral and rosette scans are performed by rotating the prisms in the same or opposite directions, respectively. Experimental measurements show an on-axis lateral resolution of 1.6μm and optical sectioning of 4.7μm, which compares with a diffraction-limited resolution of 0.8 and 1.9μm, respectively.

© 2007 Optical Society of America

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References

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2006 (2)

A. Li, L. Liu, J. Sun, X. Zhong, D. Xu, Q. Shen, Y. Zhou, Z. Luan, and L. Wang, Proc. SPIE 6304, 63041R-1 (2006).

J. Wu, M. Conry, C. Gu, F. Wang, Z. Yaqoob, and C. Yang, Opt. Lett. 31, 1265 (2006).
[CrossRef] [PubMed]

1999 (1)

G. F. Marshall, Proc. SPIE 3787, 74 (1999).
[CrossRef]

1985 (1)

1960 (1)

Appl. Opt. (1)

J. Opt. Soc. Am. (1)

Opt. Lett. (1)

Proc. SPIE (2)

G. F. Marshall, Proc. SPIE 3787, 74 (1999).
[CrossRef]

A. Li, L. Liu, J. Sun, X. Zhong, D. Xu, Q. Shen, Y. Zhou, Z. Luan, and L. Wang, Proc. SPIE 6304, 63041R-1 (2006).

Other (2)

T. WilsonConfocal Microscopy (Academic, 1990).

W. L. Wolfe and G. J. Zissis, The Infrared Handbook (Office of Naval Research, 1978).

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Figures (5)

Fig. 1
Fig. 1

Scanning concept with prisms of wedge angle α and rotation angles θ 1 and θ 2 about the optical axis. (a) A single prism deviates the beam by the vector v 1 , and (b) two prisms deviate the beam by the vector sum v 1 + v 2 . (c) A circle is scanned about the deviation from the first prism when the first prism is stationary and the second prism is rotated about the optical axis.

Fig. 2
Fig. 2

Scan patterns produced for (a) co-rotation and (b) counterrotation of the prisms at different speeds.

Fig. 3
Fig. 3

Optical layout of a confocal reflectance microscope with a dual-wedge scanner.

Fig. 4
Fig. 4

Optical sectioning measurement of 4.7 μ m with a 100 μ m pinhole.

Fig. 5
Fig. 5

Images of (a) cellulose fibers within paper and (b) the cellular structure within a plant leaf.

Equations (4)

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V j + 1 = n j n j + 1 V j + [ 1 ( n j n j + 1 ) 2 ( 1 ( V j N j ) 2 ) n j n j + 1 V j N j ] N ,
S = sin ( θ 1 θ 2 ) ,
Δ x = 0.6 n λ N A ,
Δ z = 0.95 n λ N A 2 .

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