Abstract

We present a polarization-sensitive full-field optical coherence tomography system that can produce high-resolution images of the linear retardance and reflectivity properties of biological media. En face images can be delivered at a frame rate of 3.5Hz by combination of interferometric images acquired by two CCD cameras in an interference microscope illuminated with a tungsten halogen lamp. Isotropic spatial resolution of 1.0μm is achieved. The technique is demonstrated on ex vivo muscle tissues.

© 2007 Optical Society of America

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2006

A. Dubois, G. Moneron, and A. C. Boccara, Opt. Commun. 266, 738 (2006).
[CrossRef]

N. Ugryumova, S. V. Gangnus, S. J. Matcher, Opt. Lett. 31, 2305 (2006).
[CrossRef] [PubMed]

2005

2004

2002

1998

1997

1996

A. F. Fercher, J. Biomed. Opt. 1, 157 (1996).
[CrossRef]

1992

1991

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Beaurepaire, E.

Boccara, A. C.

Bouma, B. E.

Cense, B.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Chen, T. C.

Chen, Z.

Colston, B. W.

D Silva, L. B.

de Boer, J. F.

Dubois, A.

Duncan, J.

Everett, M. J.

Fercher, A. F.

A. F. Fercher, J. Biomed. Opt. 1, 157 (1996).
[CrossRef]

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

M. R. Hee, D. Huang, E. A. Swanson, and J. G. Fujimoto, J. Opt. Soc. Am. B 9, 903 (1992).
[CrossRef]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gangnus, S. V.

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Grieve, K.

Guo, S.

Hee, M. R.

M. R. Hee, D. Huang, E. A. Swanson, and J. G. Fujimoto, J. Opt. Soc. Am. B 9, 903 (1992).
[CrossRef]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Huang, D.

M. R. Hee, D. Huang, E. A. Swanson, and J. G. Fujimoto, J. Opt. Soc. Am. B 9, 903 (1992).
[CrossRef]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Itoh, M.

Jiao, S.

Lecaque, R.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Maitland, J.

Makita, S.

Matcher, S. J.

Milner, T. E.

Moneron, G.

Mujat, M.

Nelson, J. S.

Park, B. H.

Pierce, M. C.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Schoenenberger, K.

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Sutoh, Y.

Swanson, E. A.

M. R. Hee, D. Huang, E. A. Swanson, and J. G. Fujimoto, J. Opt. Soc. Am. B 9, 903 (1992).
[CrossRef]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Tearney, G. J.

Ugryumova, N.

Vabre, L.

van Gemert, M. J. C.

Wang, L.

Wang, L. V.

Yasuno, Y.

Yatagai, T.

Yun, S. H.

Zhang, J.

Appl. Opt.

J. Biomed. Opt.

A. F. Fercher, J. Biomed. Opt. 1, 157 (1996).
[CrossRef]

J. Opt. Soc. Am. B

Opt. Commun.

A. Dubois, G. Moneron, and A. C. Boccara, Opt. Commun. 266, 738 (2006).
[CrossRef]

Opt. Express

Opt. Lett.

Science

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

Experimental arrangement. P, polarizer; BS, beam splitter (broadband, nonpolarizing); PBS, polarizing beam splitter (broadband); MO, microscope objective (water immersion, × 10 , 0.3 NA); L, achromatic doublet lens ( 300 mm focal length). The reference mirror (2% reflectivity) oscillates at 3.5 Hz . The synchronized CCD cameras are triggered at 14 Hz to acquire four images per modulation period.

Fig. 2
Fig. 2

Measured versus actual phase retardation introduced by a calibrated Babinet compensator.

Fig. 3
Fig. 3

Polarization-sensitive full-field OCT images of shrimp tail muscles. (a), (b) are en face intensity-based images acquired at depths of 30 and 100 μ m , respectively. (c), (d) are the corresponding linear-birefringence-induced phase-retardation images. The couples (a), (c) and (b), (d) illustrate the two different contrasts.

Equations (3)

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Γ = ( E 1 E 2 ) 2 + ( E 3 E 4 ) 2 ,
Γ = ( E 1 E 2 ) 2 + ( E 3 E 4 ) 2 ,
Γ R cos 2 ( ϕ ) , Γ R sin 2 ( ϕ ) ,

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