Abstract

Despite all the advances in nonlinear microscopy, all existing instruments are constrained to obtain images of one focal plane at a time. In this Letter we demonstrate a two-photon absorption fluorescence scanning microscope capable of imaging two focal planes simultaneously. This is accomplished by temporally demultiplexing the signal coming from two focal volumes at different sample depths. The scheme can be extended to three or more focal planes.

© 2007 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Simultaneous multifocal, multiphoton, photon counting microscopy

Ramón Carriles, Kraig E. Sheetz, Erich E. Hoover, Jeff A. Squier, and Virginijus Barzda
Opt. Express 16(14) 10364-10371 (2008)

Simultaneous two-plane, two-photon imaging based on spatial multiplexing

Qinglei Hu, Pei Li, Yumiao Xiong, Yu Wang, Xiaohua Lv, and Shaoqun Zeng
Opt. Lett. 43(19) 4598-4601 (2018)

In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

Wibool Piyawattanametha, Eric D. Cocker, Laurie D. Burns, Robert P. J. Barretto, Juergen C. Jung, Hyejun Ra, Olav Solgaard, and Mark J. Schnitzer
Opt. Lett. 34(15) 2309-2311 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription