Abstract

The spectroscopic content within optical coherence tomography (OCT) data can provide a wealth of information. Spectroscopic OCT methods are frequently limited by time-frequency trade-offs that limit high spectral and spatial resolution simultaneously. We present spectroscopic spectral-domain optical coherence microscopy performed with a multimodality microscope. Restricting the spatial extent of the signal by using high-numerical-aperture optics makes high-resolution spectroscopic information accessible, facilitated with spectral-domain detection. Simultaneous acquisition of multiphoton microscopy images is used to validate tissue structure and localization of nuclei within individual cells.

© 2006 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, Opt. Lett. 25, 820 (2000).
    [CrossRef]
  2. U. Morgner, W. Drexler, F. C. Kartner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, Opt. Lett. 25, 111 (2000).
    [CrossRef]
  3. C. Xu, J. Ye, D. L. Marks, and S. A. Boppart, Opt. Lett. 29, 1647 (2004).
    [CrossRef] [PubMed]
  4. D. J. Faber, E. G. Mik, M. C. Aalders, and T. G. van Leeuwen, Opt. Lett. 30, 1015 (2005).
    [CrossRef] [PubMed]
  5. D. C. Adler, T. H. Ko, P. R. Herz, and J. G. Fujimoto, Opt. Express 12, 5487 (2004).
    [CrossRef] [PubMed]
  6. C. Xu, P. S. Carney, and S. A. Boppart, Opt. Express 13, 5450 (2005).
    [CrossRef] [PubMed]
  7. C. Xu, F. Kamalabadi, and S. A. Boppart, Appl. Opt. 44, 1813 (2005).
    [CrossRef] [PubMed]
  8. D. L. Jones and T. W. Parks, IEEE Trans. Signal Process. 40, 413 (1992).
    [CrossRef]
  9. A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
    [CrossRef]
  10. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
    [CrossRef] [PubMed]
  11. R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
    [CrossRef]
  12. C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
    [CrossRef]
  13. C. Joo, T. Akkin, B. Cense, B. H. Park, and J. F. de Boer, Opt. Lett. 30, 2131 (2005).
    [CrossRef] [PubMed]
  14. A. Wax, C. Yang, and J. A. Izatt, Opt. Lett. 28, 1230 (2003).
    [CrossRef] [PubMed]
  15. Y. Liu, X. Li, Y. L. Kim, and V. Backman, Opt. Lett. 30, 2445 (2005).
    [CrossRef] [PubMed]

2006

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

2005

2004

2003

2000

1995

A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
[CrossRef]

1994

1992

D. L. Jones and T. W. Parks, IEEE Trans. Signal Process. 40, 413 (1992).
[CrossRef]

Aalders, M. C.

Adler, D. C.

Akkin, T.

Backman, V.

Boppart, S. A.

Carney, P. S.

Cense, B.

de Boer, J. F.

Drexler, W.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

U. Morgner, W. Drexler, F. C. Kartner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, Opt. Lett. 25, 111 (2000).
[CrossRef]

Elzaiat, S. Y.

A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
[CrossRef]

Faber, D. J.

Fercher, A. F.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, Opt. Lett. 25, 820 (2000).
[CrossRef]

A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
[CrossRef]

Fujimoto, J. G.

Hee, M. R.

Hermann, B.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

Herz, P. R.

Hitzenberger, C. K.

Ippen, E. P.

Izatt, J. A.

Jones, D. L.

D. L. Jones and T. W. Parks, IEEE Trans. Signal Process. 40, 413 (1992).
[CrossRef]

Joo, C.

Kamalabadi, F.

Kamp, G.

A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
[CrossRef]

Kartner, F. C.

Kim, Y. L.

Ko, T. H.

Kowalczyk, A.

Leitgeb, R.

Leitgeb, R. A.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

Li, X.

Li, X. D.

Liu, Y.

Luo, W.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Marks, D. L.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

C. Xu, J. Ye, D. L. Marks, and S. A. Boppart, Opt. Lett. 29, 1647 (2004).
[CrossRef] [PubMed]

Mik, E. G.

Morgner, U.

Owen, G. M.

Park, B. H.

Parks, T. W.

D. L. Jones and T. W. Parks, IEEE Trans. Signal Process. 40, 413 (1992).
[CrossRef]

Pitris, C.

Povazay, B.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

Ralston, T.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Sattmann, H.

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

Sticker, M.

Swanson, E. A.

Tan, W.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

van Leeuwen, T. G.

Vinegoni, C.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

Wax, A.

Wojtkowski, M.

Xu, C.

Yang, C.

Ye, J.

Appl. Opt.

Appl. Phys. Lett.

C. Vinegoni, T. Ralston, W. Tan, W. Luo, D. L. Marks, and S. A. Boppart, Appl. Phys. Lett. 88, 053901 (2006).
[CrossRef]

IEEE Trans. Signal Process.

D. L. Jones and T. W. Parks, IEEE Trans. Signal Process. 40, 413 (1992).
[CrossRef]

Opt. Commun.

A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. SPIE

R. A. Leitgeb, W. Drexler, B. Povazay, B. Hermann, H. Sattmann, and A. F. Fercher, in Proc. SPIE 5690, 151 (2005).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

(Color online) Spectral intensity curves. The arrow marks the first peak positions of the FFT of the backscattered light modulation patterns.

Fig. 2
Fig. 2

(Color online) Spectroscopic spectral-domain OCM. A, OCM of rat tissue with regions of adipose cells (middle) and muscle (upper right, lower left). B, Corresponding histology. C, SOCM image using metameric spectral analysis. D, SOCM image using LSS spectral analysis. The scale bar is representative for all images.

Fig. 3
Fig. 3

(Color online) Single-cell imaging with spectroscopic spectral-domain OCM. A, Spectral-domain OCM of a GFP-vinculin transfected fibroblast. B, Corresponding SOCM image showing localized regions of strong spectral scattering. C, Multiphoton microscopy of GFP fibroblasts colabeled with DNA–nuclear dye. D, Overlay of multiphoton DNA-dye fluorescence and OCM images.

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

I ( k , z ) z = z 0 = 2 [ R r R s ( k , z ) z = z 0 ] 1 2 S ( k ) cos ( 2 k Δ p ) ,

Metrics