Abstract

Structured illumination microscopy (SIM) is a wide-field technique that rivals confocal microscopy in optical sectioning ability at a small fraction of the acquisition time. For standard detectors such as a CCD camera, SIM requires a minimum of three sequential frame captures, limiting its usefulness to static objects. By using a color grid and camera, we surpass this limit and achieve optical sectioning with just a single image acquisition. The extended method is now applicable to moving objects and improves the speed of three-dimensional imaging of static objects by at least a factor of three.

© 2006 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Optically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processing

Krzysztof Patorski, Maciej Trusiak, and Tomasz Tkaczyk
Opt. Express 22(8) 9517-9527 (2014)

Multi-view object topography measurement with optical sectioning structured illumination microscopy

Feifei Ren, Zhaojun Wang, Jia Qian, Yansheng Liang, Shipei Dang, Yanan Cai, Piero R. Bianco, Baoli Yao, and Ming Lei
Appl. Opt. 58(23) 6288-6294 (2019)

Quantitative sectioning and noise analysis for structured illumination microscopy

Nathan Hagen, Liang Gao, and Tomasz S. Tkaczyk
Opt. Express 20(1) 403-413 (2012)

More Recommended Articles

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (9)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription