Abstract

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

© 2006 Optical Society of America

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References

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  1. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
    [CrossRef] [PubMed]
  2. E. Wolf, Opt. Commun. 1, 153 (1969).
    [CrossRef]
  3. R. Dändliker and K. Weiss, Opt. Commun. 1, 323 (1970).
    [CrossRef]
  4. V. Lauer, J. Microsc. 205, 165 (2002).
    [CrossRef] [PubMed]
  5. A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
    [CrossRef]
  6. T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
    [PubMed]
  7. P. Marquet, M.D./Ph.D. dissertation (UNI-Lausanne, 2003), Chap. 5.
  8. A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (Society for Industrial and Applied Mathematics, 2001), chap. 3.
    [CrossRef]
  9. T. C. Wedberg, J. J. Stamnes, and W. Singer, Appl. Opt. 34, 6575 (1995).
    [CrossRef] [PubMed]
  10. C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
    [CrossRef]
  11. T. Noda, S. Kawata, and S. Minami, Appl. Opt. 31, 670 (1992).
    [CrossRef] [PubMed]
  12. A. Dunn, "Light scattering properties of cells," Ph.D. dissertation (University of Texas, Austin, 1997), chap. 1.

2005

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
[CrossRef] [PubMed]

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

2002

V. Lauer, J. Microsc. 205, 165 (2002).
[CrossRef] [PubMed]

2000

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

1995

1992

1970

R. Dändliker and K. Weiss, Opt. Commun. 1, 323 (1970).
[CrossRef]

1969

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

Allman, B. E.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Aspert, N.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Barty, A.

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

Bellair, C. J.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Charrière, F.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Colomb, T.

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
[CrossRef] [PubMed]

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Cuche, E.

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
[CrossRef] [PubMed]

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Curl, C. L.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Dändliker, R.

R. Dändliker and K. Weiss, Opt. Commun. 1, 323 (1970).
[CrossRef]

Delbridge, L. M.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Depeursinge, C.

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
[CrossRef] [PubMed]

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Dunn, A.

A. Dunn, "Light scattering properties of cells," Ph.D. dissertation (University of Texas, Austin, 1997), chap. 1.

Emery, Y.

Harris, P. J.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Harris, T.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Kak, A. C.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (Society for Industrial and Applied Mathematics, 2001), chap. 3.
[CrossRef]

Kawata, S.

Kühn, J.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Lauer, V.

V. Lauer, J. Microsc. 205, 165 (2002).
[CrossRef] [PubMed]

Magistretti, P. J.

Marquet, P.

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
[CrossRef] [PubMed]

P. Marquet, M.D./Ph.D. dissertation (UNI-Lausanne, 2003), Chap. 5.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Minami, S.

Montfort, F.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

Noda, T.

Nugent, K. A.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

Paganin, D.

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

Rappaz, B.

Roberts, A.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

Singer, W.

Slaney, M.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (Society for Industrial and Applied Mathematics, 2001), chap. 3.
[CrossRef]

Stamnes, J. J.

Stewart, A. G.

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

Wedberg, T. C.

Weiss, K.

R. Dändliker and K. Weiss, Opt. Commun. 1, 323 (1970).
[CrossRef]

Wolf, E.

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

Appl. Opt.

T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberrations compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. (to be published).
[PubMed]

T. C. Wedberg, J. J. Stamnes, and W. Singer, Appl. Opt. 34, 6575 (1995).
[CrossRef] [PubMed]

T. Noda, S. Kawata, and S. Minami, Appl. Opt. 31, 670 (1992).
[CrossRef] [PubMed]

Cytometry Part A

C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, Cytometry Part A 65, 88 (2005).
[CrossRef]

J. Microsc.

V. Lauer, J. Microsc. 205, 165 (2002).
[CrossRef] [PubMed]

Opt. Commun.

A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, Opt. Commun. 175, 329 (2000).
[CrossRef]

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

R. Dändliker and K. Weiss, Opt. Commun. 1, 323 (1970).
[CrossRef]

Opt. Lett.

Other

A. Dunn, "Light scattering properties of cells," Ph.D. dissertation (University of Texas, Austin, 1997), chap. 1.

P. Marquet, M.D./Ph.D. dissertation (UNI-Lausanne, 2003), Chap. 5.

A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (Society for Industrial and Applied Mathematics, 2001), chap. 3.
[CrossRef]

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Figures (2)

Fig. 1
Fig. 1

Holographic microscope for transmission imaging: NF, neutral-density filter; PBS, polarizing beam splitter; BE, beam expander with spatial filter; λ 2 , half-wave plate; MO, microscope objective; M, mirror; BS, beam splitter; O, object wave; R, reference wave; MP, micropipette; S, specimen; IML, index matching liquid. Inset: detail showing the off-axis geometry at the incidence on the CCD.

Fig. 2
Fig. 2

Tomography of a pollen cell RI: (1) cut along the x y plane in the middle of the pollen cell (2), cuts at different positions in the cell along the y z plane, and (3) the x z plane, and (4) schematic of the presented cuts.

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