For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.
© 2006 Optical Society of AmericaFull Article | PDF Article
Pengyi Guo and Anthony J. Devaney
J. Opt. Soc. Am. A 22(11) 2338-2347 (2005)
Florian Charrière, Nicolas Pavillon, Tristan Colomb, Christian Depeursinge, Thierry J. Heger, Edward A.D. Mitchell, Pierre Marquet, and Benjamin Rappaz
Opt. Express 14(16) 7005-7013 (2006)
Frédéric Montfort, Tristan Colomb, Florian Charrière, Jonas Kühn, Pierre Marquet, Etienne Cuche, Sylvain Herminjard, and Christian Depeursinge
Appl. Opt. 45(32) 8209-8217 (2006)